Mass Spectrometry Imaging: Principles and Protocols

by Stanislav S. Rubakhin (Editor), Jonathan V. Sweedler (Editor)

Overview

Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Prools, expert practitioners from academia, industry, and the clinic contribute cutting-edge prools describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative prools to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology™ series format, prool chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting. Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Prools is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source.

Product Details

ISBN-13:	9781607617457
Publisher:	Springer-Verlag New York, LLC
Publication date:	08/06/2010
Series:	Methods in Molecular Biology , #656
Edition description:	2010
Pages:	490
Product dimensions:	7.20(w) x 10.20(h) x 1.40(d)

Table of Contents

Preface v

Contributors xi

Part I Introduction Reviews and Tutorials

1 Imaging Mass Spectrometry: Viewing the Future Sarah A. Schwartz Richard M. Caprioli 3

2 A Mass Spectrometry Primer for Mass Spectrometry Imaging Stanislav S. Rubakhin Jonathan V. Sweedler 21

3 Imaging of Metals, Metalloids, and Non-Metals by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in Biological Tissues J. Sabine Becker J. Susanne Becker 51

Part II Protocols for MS Imaging of Distribution of Small Molecules Including Metabolites and Pharmaceuticals

4 Lipid Detection, Identification, and Imaging Single Cells with SIMS Michael L. Heien Paul D. Piehowski Nicholas Winograd Andrew G. Ewing 85

5 The Application and Potential of Ion Mobility Mass Spectrometry in Imaging MS with a Focus on Lipids Amina S. Woods Shelley N. Jackson 99

6 Quantitative Imaging of Chemical Composition in Single Cells by Secondary Ion Mass Spectrometry: Cisplatin Affects Calcium Stores in Renal Epithelial Cells Subhash Chandra 113

7 Imaging MALDI Mass Spectrometry of Sphingolipids Using an Oscillating Capillary Nebulizer Matrix Application System Yanfeng Chen Ying Liu Jeremy Allegood Elaine Wang Begoña Cachón-González Timothy M. Cox Alfred H. Merrill M. Cameron Sullards 131

8 Mapping Pharmaceuticals in Rat Brain Sections Using MALDI Imaging Mass Spectrometry Yunsheng Hsieh Fangbiao Li Walter A. Korfmacher 147

9 Laser Ablation Electrospray Ionization for Atmospheric Pressure Molecular Imaging Mass Spectrometry Peter Nemes Akos Vertes 159

10 Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Small Metabolites: A Practical Protocol Yuki Sugiura Mitsutoshi Setou 173

11 Cellular Imaging Using Matrix-Enhanced and Metal-Assisted SIMS A.F. Maarten Altelaar Sander R. Piersma 197

12 Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue Timothy J. Garrett Richard A. Yost 209

13 Chemical Imaging with Desorption Electrospray Ionization Mass Spectrometry Vilmos Kertesz Gary J. Van Berkel 231

14 Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) Qiang Lin Yongsheng xiao Lin He 243

15 Preparation of Single Cells for Imaging Mass Spectrometry Elena S.F. Berman Susan L. Fortson Kristen S. Kulp 253

16 Applying Imaging ToF-SIMS and PCA in Differentiation of Tissue Types Ligang Wu James S. Felton Kuang Jen J. Wu 267

Part III Protocols for MS Imaging of Distribution of Peptides and Proteins

17 Direct Molecular Analysis of Whole-Body Animal Tissue Sections by MALDI Imaging Mass Spectrometry Michelle L. Reyzer Pierre Chaurand Peggi M. Angel Richard M. Caprioli 285

18 MALDI Direct Analysis and Imaging of Frozen Versus FFPE Tissues: What Strategy for Which Sample? Maxence Wisztorski Julien Franck Michel Salzet Isabelle Fournier 303

19 On Tissue Protein Identification Improvement by N. Terminal Peptide Derivatization Julien Franck Mohamed El Ayed Maxence Wisztorski Michel Salzet Isabelle Fournier 323

20 Specific MADI-MSI:TAG-MASS Jonathan Stauber, Mohamed El Ayed Maxence Wisztorski Michel Salzet Isabelle Fournier 339

21 Structually Selective Imaging Mass Spectrometry by Imaging Ion Mobility Mass Spectrometry John A. McLean Larissa S. Fenn Jeffrey R. Enders 363

22 Tutorial: Multivariate Statistical Treatment of Imaging Data for Clinical Biomarker Discovery Sören-Oliver Deininger Michael Becker Detlev Suckau 385

23 Applications of MALDI-MSI to Pharmaceutical Research Brendan Prideaux Dieter Staab Markus Stoeckli 405

24 Tissue Preparation for the In Situ MALDI MS Imaging of Proteins, Lipids, and Small Molecules at Cellular Resolution Nathalie Y.R. Agar Jane-Marie Kowalski Paul J. Kowalski John H. Wong Jeffrey N. Agar 415

25 Imaging of Similar Mass Neuropeptides in Neuronal Tissue by Enhanced Resolution MALDI MS with an Ion Trap - Orbitrap? Hybrid Instrument Peter D.E.M. Verhaert Martijn W.H. Pinkse Kerstin Strupat Maria C. Prieto Conaway 433

26 Mass Spectrometric Imaging of Neuropeptides in Decapod Crustacean Neuronal Tissues Ruibing Chen Stephanie S. Cape Robert M. Sturm Lingjun Li 451

27 Mass Spectrometry Imaging Using the Stretched Sample Approach Tyler A. Zimmerman Stanislav S. Rubakhin Jonathan V. Sweedler 465

Subject Index 481

Editorial Reviews

From the reviews:

“The book aims to provide comprehensive background information on mass spectrometry imaging (MSI) technology … . a monograph that has the merit of summarizing many prools of great value for all scientists, engineers, and clinicians who are interested in applying MSI in their work. The book is clearly written and comprehensive, and has a good balance between theoretical descriptions and broad practical descriptions. Both established MSI researchers and those new to the field would benefit from this valuable source of methodological information.” (José Manuel Costa-Fernández, Analytical and Bioanalytical Chemistry, Vol. 401, 2011)

From the Publisher