Methicillin-Resistant Staphylococcus aureus (MRSA) Protocols / Edition 1

by Yinduo Ji

Overview

MRSA Prools for Methods in Molecular Biology provides a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA. Each chapter begins with a brief introduction to the method and purpose and then goes into detailed prools for every step of analysis. Several chapters also include a section with tips on individual steps not usually found in methods books. These tips may represent the difference between immediate success and lengthy troubleshooting.

Among the topics discussed in this volume are Staphylococcus aureus infections, MIC analysis, ITS-PCR identification, SCCmec analysis, and Biofilm formation. The book also contains helpful case studies, and authors provide insights into the control, prevention, and treatment of infections.

Oriented towards routine clinical diagnosis, research, and actual practice for treatment of patients infected by MRSA, MRSA Prools for Methods in Molecular Biology will make a useful basis for anyone interested in instituting a new method for studying MRSA.

Product Details

ISBN-13:	9781617376986
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/10/2010
Series:	Methods in Molecular Biology , #391
Edition description:	Softcover reprint of hardcover 1st ed. 2007
Pages:	264
Product dimensions:	6.00(w) x 8.90(h) x 0.80(d)

Table of Contents

Clinical, Epidemiological, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus (MRSA) Infections.- MRSA Case Studies.- Minimum Inhibitory Concentration (MIC) Analysis and Susceptibility Testing of MRSA.- Internal Transcribed Spacer (ITS)-PCR Identification of MRSA.- Pulsed-Field Gel Electrophoresis of MRSA.- Multilocus Sequence Typing (MLST) of Staphylococcus aureus.- Staphylococcal Cassette Chromosome mec (SCCmec) Analysis of MRSA.- Targeted Gene Disruption for the Analysis of Virulence of Staphylococcus aureus 1.- Molecular Analysis of Staphylococcal Superantigens.- Investigation of Biofilm Formation in Clinical Isolates of Staphylococcus aureus.- Comparative Analysis of Staphylococcal Adhesion and Internalization by Epithelial Cells.- Comparative Analysis of MRSA.- Genomic Analysis of Gene Expression of Staphylococcus aureus.- Proteomic Approach to Investigate MRSA.- Environmental Surveillance for MRSA.- Control and Prevention of MRSA Infections.- Treatment of Infections Caused by Resistant Staphylococcus aureus.

Editorial Reviews

From the reviews:

"This book … has a unique format focussing on detailed experimental prools. This is particularly useful if you are trying a technique for the first time, troubleshooting a method, or wishing to understand how a technique works in order to critique its use in the literature. … there is much to recommend in this book, particularly those who are new to the field and want an introduction to techniques in both basic biology and clinical aspects of MRSA." (Jodi Lindsay, Microbiology Today, May, 2008)

"The book is devoted to the multiple laboratory techniques available for technicians and basic scientists. … a good field manual, and clinicians will have an easy to read book on these techniques. … describes well the laboratory techniques available for the study of MRSA. … Most chapters have illustrations in black and white, which are helpful in the overall discussion, and all chapters have a bibliographic list. … it is a good starting point for clinicians and novice scientists in the world of MRSA infections." (Carlos Ernesto Figueroa Castro, Doody’s Review Service, May, 2008)

"A unique compilation of 17 chapters aimed chiefly at the scientific investigation of MRSA … . This book may also serve as a classic methods reference for those geared towards studying MRSA … . In summary, this book represents an excellent reference manual of prools used to study MRSA, with concise, clear, and well-written introductory sections that can be useful for laboratory investigators and clinicians wishing to get a better understanding of the theory behind scientific methods. … is timely in its arrival." (George Sakoulas, Clinical Infectious Diseases, Vol. 46, May, 2008)

From the Publisher

Reviewer: Carlos E Figueroa Castro, MD (Medical College of Wisconsin)
Description: This addition to the Methods in Molecular Biology series has some chapters on clinical management of MRSA infections, but the main emphasis is on the description of the wide array of laboratory methods available for basic and clinical research on MRSA.
Purpose: Based on preface, the purpose of the authors is "to provide a comprehensive collection of the most up-to-date techniques for the detection and investigation of MRSA." Given the diversity of the techniques described, the purpose has been fulfilled.
Audience: Some chapters cover the clinical and epidemiological aspects of MRSA infection, but the majority of the book is devoted to the multiple laboratory techniques available for technicians and basic scientists. The last group will have a good field manual, and clinicians will have an easy to read book on these techniques. In that sense, the book has two main audiences.
Features: From a clinician's perspective, the book describes well the laboratory techniques available for the study of MRSA. With the increasing problem of MRSA infections, and the help derived from these techniques, clinicians will feel comfortable discussing the basic aspects of the science used for research on MRSA. I found the descriptions on pulse-field gel electrophoresis, multilocus sequence typing, and staphylococcal cassette chromosome analysis very useful. These techniques are used widely in clinical research, and are a good starting point to understanding the literature. In addition, the use of these techniques will be extended to the daily practice in the near future. Other chapters, like biofilm formation and proteomic analysis, are interesting and might be an introduction to a more formal training for a neophyte. Most chapters have illustrations in black and white, which are helpful in the overall discussion, and all chapters have a bibliographic list. It would be a good idea to share the contents of this book with your laboratory section. The field of molecular biology is a moving target, and new techniques or improvement of old methods are incorporated on a daily basis. The clinical chapters try to put things in perspective, but clinicians could find better sources for the medical management of patients with MRSA infections.
Assessment: This is not an all inclusive book on the clinical and laboratory management of MRSA infections, but it is a good starting point for clinicians and novice scientists in the world of MRSA infections.

Doody's Review Service