Microchip Capillary Electrophoresis: Methods and Protocols / Edition 1

by Charles Henry

Overview

Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Product Details

ISBN-13:	9781617374760
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/10/2010
Series:	Methods in Molecular Biology , #339
Edition description:	Softcover reprint of hardcover 1st ed. 2006
Pages:	238
Product dimensions:	5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

Part I. Microchip Fabrication Methods

Fabrication of a Glass Capillary Electrophoresis Microchip With Integrated Electrodes
Mark M. Crain, Robert S. Keynton, Kevin M. Walsh, Thomas J. Roussel, Jr., Richard P. Baldwin, John F. Naber, and Douglas J. Jackson

Micro-Molding for Poly(dimethylsiloxane) Microchips
Carlos D. García and Charles S. Henry

Fabrication of Polymer Microfluidic Systems by Hot Embossing and Laser Ablation
Laurie E. Locascio, David J. Ross, Peter B. Howell, and Michael Gaitan

Part II. Surface Modification Methods

Surface Modification Methods for Enhanced Device Efficacy and Function
Barbara J. Jones and Mark A. Hayes

Polyelectrolyte Coatings for Microchip Capillary Electrophoresis
Yan Liu and Charles S. Henry

Part III. Detection Methods for Microchip Capillary Electrophoresis

Interfacing Microchip Capillary Electrophoresis With Electrospray Ionization Mass Spectrometry
Trust Razunguzwa and Aaron T. Timperman

Interfacing Amperometric Detection With Microchip Capillary Electrophoresis
R. Scott Martin

Conductivity Detection on Microchips
Roland Hergenröder and Benedikt Graß

Part IV. Applications of Microchip Capillary Electrophoresis

DNA Separations
Andrea W. Chow

Protein Separations
Andrea W. Chow

Microchip Capillary Electrophoresis: Application to Peptide Analysis
Barbara A. Fogarty, Nathan A. Lacher, and Susan M. Lunte

Measuring Electroosmotic Flow in Microchips and Capillaries
S. Douglass Gilman and Peter J. Chapman

Single Cell Analysis on Microfluidic Devices
Christopher T. Culbertson

Rapid DNA Amplification in Glass Microdevices
Christopher J. Easley, Lindsay A. Legendre, James P. Landers, and Jerome P. Ferrance

Index

Editorial Reviews

"From this book the reader can acquire a basic command of the technique relatively fast and, if required, can move on to in-depth fundamental, or specific information in the literature of this explosively growing area of science." -Molecular Biotechnology

From the Publisher