Mitochondrial Bioenergetics: Methods and Protocols

by Carlos M. Palmeira (Editor), António J. Moreno (Editor)

Overview

This second edition volume expands on the previous edition with chapters discussing the latest developments and research initiatives in mihondrial functions. The chapters in this book explore topics such as high-resolution fluorespirometry and OXPHOS prools in human cells, analysis of mihondrial oxygen consumption, mihondrial bioenergetics, and mihondrial dynamics in mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Mihondrial Bioenergetics: Methods and Prools, Second Edition is a valuable resource for students, and practitioners in the pharmaceutical sciences, environmental sciences, and mihondrial genetics industries.

Product Details

ISBN-13:	9781493992980
Publisher:	Springer New York
Publication date:	07/25/2019
Series:	Methods in Molecular Biology , #1782
Edition description:	Second Edition 2018
Pages:	422
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Overview of Mihondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mihondria and Permeabilized Animal Cells.- High-Resolution FluoRespirometry and OXPHOS Prools for Human Cells, Permeabilized Fibres from Small Biopsies of Muscle and Isolated Mihondria.- High-Throughput Analysis of Mihondrial Oxygen Consumption.- Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices.- Mihondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mihondria.- Fluorescence Measurement of Mihondrial Membrane Potential Changes in Cultured Cells.- Comparison of Mihondrial Incubation Media for Measurement of Respiration and Hydrogen Peroxide Production.- Measurement of Proton Leak in Isolated Mihondria.- Imaging Mihondrial Calcium Fluxes with Fluorescent Probes and Single or Two Photon Confocal Microscopy.- Mihondrial Permeability Transition Pore and Calcium Handling.- Redox Equivalents and Mihondrial Bioenergetics.- Mihondrial Bioenergetics by 13 C-NMR Isotopomer Analysis.- Computational Modeling of Mihondrial Function from a Systems Biology Perspective.- Monitoring the Mihondrial Dynamics in Mammalian Cells.- Plate-Based Measurement of Superoxide and Hydrogen Peroxide Production by Isolated Mihondria.- Plate-Based Measurement of Respiration by Isolated Mihondria.- Detection of Iron Depletion- and Hypoxia-Induced Mitophagy in Mammalian Cells.- The Importance of Calcium Ions for Determining Mihondrial Glycerol-3-Phosphate Dehydrogenase Activity When Measuring Uncoupling Protein 1 (UCP1) Function in Mihondria Isolated from Brown Adipose Tissue.- Isolation and Analysis of Mihondrial Small RNAs from Rat Liver Tissue and HepG2 Cells.- Imaging of Mihondrial pH Using SNARF-1.- Relation between Mihondrial Membrane Potential and ROS Formation.- Assessing Spatio-Temporal and Functional Organization of Mihondrial Networks.- Measurement of Mihondrial ROS Formation.