Molecular Genetics of Asthma

by Marïa Isidoro Garcïa (Editor)

Overview

Presenting topics from the basic application of molecular genetics to more complex gene expression analysis using different models of study, this detailed volume explores asthma through the lens of genetics, considered to play an essential role in the etiopathogenesis of the disease. Since asthma is a complex disease, this book is designed to provide a review of the most useful techniques with examples of their applications in specific laboratory prools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Molecular Genetics of Asthma serves as an ideal guide to researchers investigating this vital field of study.

Product Details

ISBN-13:	9781493981076
Publisher:	Springer New York
Publication date:	05/30/2018
Series:	Methods in Molecular Biology , #1434
Edition description:	Softcover reprint of the original 1st ed. 2016
Pages:	275
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Applications of Molecular Genetics to the Study of Asthma.- Assessment and Validation of New Genetic Variants: A systematic In Silico Approach.- Introduction to the Gene Expression Analysis.- Real Time PCR for Gene Expression Quantification in Asthma.- Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma.- Review of Methods to Study Gene Expression Regulation Applied to Asthma.- Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma.- SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma.- Chromatin Immunoprecipitation: Application to the Study of Asthma.- Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Prools.- Prools for Exosome Isolation and RNA Profiling.- Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell.- Prool for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line.- Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line.- Review of Mouse Models Applied to the Study of Asthma.- General and Specific Mouse Models for Asthma Research.- Mouse Models applied to the Research of Pharmacological Treatments in Asthma.- Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma.