This multiauthored text contains a collection of established laboratory protocols in clinical molecular pathology used for the diagnosis and monitoring cancer, infectious diseases, and genetic disorders. It contains cutting-edge molecular diagnostic techniques with detailed step-by-step instructions and tips to obtain successful results. Since many molecular pathological techniques are only to be found in the primary literatures, the purpose here is to offer readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. It is a worthy objective and the editor meets it very well. According to the editor, this book is intended for clinical laboratory use by medical technologists and pathologists. It will also be a good reference book for the researchers who are interested in or already performing these assays. Each protocol in this book includes sections of introduction, materials, methods, and notes. Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology. Most protocols use polymerase chain reaction (PCR) based techniques. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays. This book has good references in both background of diseases and techniques. Most charts and tables have high quality; however, some figures are of poor or low quality, especially those color photos. Somefigures are not labeled well. The significant diagnostic utility of molecular protocols in cancer, hematological malignancies, and virus related diseases is covered well. This book is relatively limited in the diagnosis of inherited diseases. This book is well written and the molecular protocols introduced can be easily followed practically. It is a good protocol resource book in molecular pathology.
US, Canadian, and Finnish molecular pathologists set out 35 readily reproducible laboratory techniques that either are frequently performed or are recognized for their significant diagnostic utility. The methods, detailed step-by-step, can be used to diagnose or monitor cancer, hematological malignancies, infectious diseases, and selected genetic disorders. The collection represents a step toward standardization in the rapidly developing field. Among the techniques presented are extracting DNA from fresh or frozen tissues, detecting telomerase by hybridization and by the polymerase chain reaction-based telomerase activity assay, and detecting and speciating mycobacteria in formalin-fixed paraffin-embedded tissue sections. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Reviewer: Yu Li, MD (University of Kansas Medical Center)
Description: This multiauthored text contains a collection of established laboratory protocols in clinical molecular pathology used for the diagnosis and monitoring cancer, infectious diseases, and genetic disorders. It contains cutting-edge molecular diagnostic techniques with detailed step-by-step instructions and tips to obtain successful results.
Purpose: Since many molecular pathological techniques are only to be found in the primary literatures, the purpose here is to offer readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. It is a worthy objective and the editor meets it very well.
Audience: According to the editor, this book is intended for clinical laboratory use by medical technologists and pathologists. It will also be a good reference book for the researchers who are interested in or already performing these assays.
Features: Each protocol in this book includes sections of introduction, materials, methods, and notes. Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology. Most protocols use polymerase chain reaction (PCR) based techniques. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays. This book has good references in both background of diseases and techniques. Most charts and tables have high quality; however, some figures are of poor or low quality, especially those color photos. Some figures are not labeled well. The significant diagnostic utility of molecular protocols in cancer, hematological malignancies, and virus related diseases is covered well. This book is relatively limited in the diagnosis of inherited diseases.
Assessment: This book is well written and the molecular protocols introduced can be easily followed practically. It is a good protocol resource book in molecular pathology.
"It contains cutting-edge molecular diagnostic techniques with detailed step-by-step instructions and tips to obtain successful results....the purpose here is to offer readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. It is a worthy objective and the editor meets it very well....Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology....Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays....The significant diagnostic utility of molecular prools in cancer, hermatological malignancies, and virus related diseases is covered well....It is a good prool resource book in molecular pathology."-Doody's Health Sciences Book Review Journal
"Molecular Pathology Prools fills a unique niche for general molecular pathology laboratories that may be called upon to perform a wide variety of tests for infectious and noninfectious diseases. It has 35 chapters that provide extensive detail on laboratory prools for a variety of important conditions, from techniques for preparation of nucleic acid templates and mutation detection for genetic and neoplastic evaluations, to hematological applications, to a limited number of detailed prools for infectious disease diagnosis and monitoring...The major strength of the prools discussed in this book lies in their enormous detail and the practical remarks of the authors, which will assist in establishing the assays in a new laboratory. Another great value in some chapters is the emphasis on the assessment of quality control that will meet or exceed all the requirements of agencies that license and regulate clinical laboratories." - Clinical Infectious Diseases
"A must for genetic laboratories and all those using the new molecular biological methods." - Journal of Endocrine Genetics
"Each prool in this book includes sections of introduction, materials, methods, and notes. Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology. Most prools use polymerase chain reaction (PCR) based techniques. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in sity hybridization assays. This book has good references in both background of diseases and techniques. Most charts and labels have high quality;...The significant diagnostic utility of molecular prools in cancer, hematological malignancies, and virus related diseases is covered well....This book is well written and the molecular prools introduced can be easily followed practically." - E-Streams
