Monooxygenase, Peroxidase and Peroxygenase Properties and Mechanisms of Cytochrome P450

by Eugene G. Hrycay (Editor), Stelvio M. Bandiera (Editor)

Overview

This book describes in 13 chapters mechanisms of P450 used to monooxygenate substrates via the NAD(P)H/O₂ pathway using its peroxidase and peroxygenase functions. P450 also utilizes peroxides, peracids, periodate and iodosobenzene to oxygenate substrates via the shunt pathway. Also described are mechanisms used in the oxidation of pharmaceuticals by CYP3A4; acyl- carbon cleavage by CYP17A1, CYP19A1 and CYP51A1; metabolism of tetrabromodiphenyl ethers and bile acids by CYP2B6 and CYP3A4; metabolism of ω-6 and ω-3 polyunsaturated fatty acids; H₂O₂-mediated peroxygenation of substrates using substrate misrecognition; P450 oxidative reactions using electrochemical methods; electron transfer to P450 by redox proteins; hydroxylation of 1,8-cineole by P450cin; and peroxygenation by unspecific peroxygenases using H₂O₂. The topics covered are relevant to P450 researchers, professors and students from a variety of disciplines ranging from pharmacology, toxicology and microbiology to chemistry.

Product Details

ISBN-13:	9783319160085
Publisher:	Springer International Publishing
Publication date:	05/24/2015
Series:	Advances in Experimental Medicine and Biology , #851
Edition description:	2015
Pages:	368
Product dimensions:	7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

Foreword.- 1. Monooxygenase, Peroxidase and Peroxygenase Properties and Reaction Mechanisms of Cyhrome P450 Enzymes .- 2. Oxidizing Intermediates in P450 Catalysis: A Case for Multiple Oxidants.- 3. Current Approaches for Investigating and Predicting Cyhrome P450 3A4-Ligand Interactions.- 4. Acyl-Carbon Bond Cleaving Cyhrome P450 Enzymes: CYP17A1, CYP19A1 and CYP51A1.- 5. Regioselective Versatility of Monooxygenase Reactions Catalyzed by CYP2B6 and CYP3A4: Examples With Single Substrates.- 6. Cyhrome P450 Enzymes in the Bioactivation of Polyunsaturated Fatty Acids and their Role in Cardiovascular Disease.- 7. Monooxygenation of Small Hydrocarbons Catalyzed by Bacterial Cyhrome P450s.- 8. Use of Chemical Auxiliaries to Control P450 Enzymes for Predictable Oxidations at Unactivated C-H Bonds of Substrates,- 9. Cyhrome P450 Enzymes and Electrochemistry: Crosstalk with Electrodes as Redox Partners and Electron Sources.- 10. Mechanistic Basis of Electron Transfer to Cyhromes P450 by Natural Redox Partners and Artificial Donor Constructs .- 11. Biological Diversity of Cyhrome P450 Redox Partner Proteins .- 12. Cyhrome P450_cin (CYP176A1).- 13. Fungal Unspecific Peroxygenases: Heme-Thiolate Proteins that Combine Peroxidase and Cyhrome P450 Properties.