New and Emerging Proteomic Techniques

by Dobrin Nedelkov (Editor), Randall W. Nelson (Editor)

Overview

Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The prools follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Product Details

ISBN-13:	9781588295194
Publisher:	Springer-Verlag New York, LLC
Publication date:	03/01/2006
Series:	Methods in Molecular Biology , #328
Edition description:	2006
Pages:	248
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

On-Chip Protein Synthesis for Making Microarrays.- RCA-Enhanced Protein Detection Arrays.- Antibody Microarrays Using Resonance Light-Scattering Particles for Detection.- Chemical Proteomics Profiling of Proteasome Activity.- Two-Dimensional Difference Gel Electrophoresis.- Oligomeric States of Proteins Determined by Size-Exclusion Chromatography Coupled With Light Scattering, Absorbance, and Refractive Index Detectors.- Surface Plasmon Resonance Imaging Measurements of Protein Interactions With Biopolymer Microarrays.- Surface Plasmon Resonance Mass Spectrometry for Protein Analysis.- High-Throughput Affinity Mass Spectrometry.- Isotope-Coded Affinity Tags for Protein Quantification.- Proteomic Analysis by Multidimensional Protein Identification Technology.- Isolation of Glycoproteins and Identification of Their N-Linked Glycosylation Sites.- N-Glycosylation Analysis Using the StrOligo Algorithm.- MALDI-MS Data Analysis for Disease Biomarker Discovery.- Using the Global Proteome Machine for Protein Identification.

Editorial Reviews

From the reviews:

"It is no surprise that a book of this title is somewhat eclectic. … Methodologies are well written and useful, emphasizing that proteins and function can be studied at differing levels of resolution. … this book will be useful for beginners, though it is really more for the initiated seeking inspiration." (Dr. Mike J. Naldrett, Microbiology Today, November, 2006)

“New and Emerging Proteomics Techniques contains detailed descriptions of modern proteomics techniques written by experts in the field. … Overall, the book’s background information and detailed prools and notes would be of interest to researchers in many areas who are interested in knowing more about the types of studies now made possible by these powerful, combined or individual proteomics techniques, and how they might be applied to current questions in basic and medical science.”­­­ (Constance J. Jeffery, Journal of the American Society for Mass Spectrometry, Vol. 18, 2007)

From the Publisher