PCR Protocols / Edition 3

by Daniel J. Park

Overview

Known for flexibility and robustness, PCR techniques continue to improve through numerous developments, including the identification of thermostable DNA polymerases which exhibit a range of properties to suit given applications. PCR Prools, Third Edition selects recently developed tools and tricks, contributed by field-leading authors, for the significant value that they add to more generally established methods. Along with the cutting-edge methodologies, this volume describes many core applications, such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, ‘DNA fingerprinting’, diagnostics, protein engineering, interaction screening as well as a chapter highlighting workflow considerations and contamination control, crucial for all PCR methods. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary reagents and materials, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, PCR Prools, Third Edition seeks to further elucidate this essential technique while also providing core principles with broad applications for scientists of all backgrounds.

Product Details

ISBN-13:	9781627038195
Publisher:	Springer-Verlag New York, LLC
Publication date:	10/01/2014
Series:	Methods in Molecular Biology , #687
Edition description:	3rd ed. 2011
Pages:	348
Product dimensions:	6.69(w) x 9.61(h) x (d)

Table of Contents

Setup of a PCR Laboratory.- Long Range PCR with a DNA Polymerase Fusion.- Isolation of Genomic Insertion Sites of Proviruses Using Splinkerette-PCR-Based Procedures.- Lariat-Dependent Nested PCR for Flanking Sequence Determination.- CODEHOP PCR and CODEHOP PCR Primer Design.- Sequencing of Difficult DNA Regions by SAM Sequencing.- A Global Single-Cell cDNA Amplification Method for Quantitative Microarray Analysis.- Quantitation of MicroRNAs by Real-Time RT-qPCR.- High-Throughput SuperSAGE.- Deep Cap Analysis of Gene Expression (Deep CAGE).- Linking Emulsion PCR Haplotype Analysis.- PAP-LMPCR: An Improved, Sequence-Selective Method for the In vivo Analysis of Transcription Factor Occupancy and Chromatin Fine Structure.- The Many Faces of MLPA.- Assessing Gene-Specific Methylation Using HRM-Based Analysis.- Alu PCR.- Asynchronous PCR.- Novel Applications of PCR through the Use of DNA Substrates.- Enhanced Solid Phase PCR for Increased Loading of Amplicon onto Solid Support.- Application of Blocking Oligonucleotides to Improve Signal to Noise Ratio in a PCR.- Asymmetric Overlap Extension PCR Method for Site-Directed Mutagenesis.- Ribosome Display: A Technology for Selecting and Evolving Proteins from Large Libraries.- GLOBE: Analysis of DNA-Protein Interaction Analysis.- PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues.- Nucleotide Exchange and Excision Technology (NExT) DNA Shuffling and Directed Evolution.

Editorial Reviews

Reviewer: Omer Iqbal, MD, FACC, FESC (Loyola University Medical Center)
Description: This book describes recently developed tools such as PCR cloning and sequencing, expression, copy number or methylation profile analysis, DNA fingerprinting, diagnostics, protein engineering interaction screening, and other important techniques crucial for all PCR methods.
Purpose: The purpose is to provide scientists with latest tools necessary for PCR methods.
Audience: Students and researchers interested in learning latest methods in PCR will find this book extremely useful.
Features: The book is divided into five parts. Part I covers PCR husbandry and includes an interesting chapter on the setup of a PCR laboratory. Part II covers cloning and sequencing, part III, expression, epigenetic and copy number analysis, part IV, probes and assays, and part V, engineering and screening. Each chapter uses the same format: an introduction, lists of necessary reagents and materials, step-by-step readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Assessment: This book presents cutting-edge methodologies crucial for all PCR methods. Given the rapid advances in the field of PCR methods, this third edition is essential and justifies replacement of the previous edition.

Doody's Review Service

From the reviews of the third edition:

“This book describes recently developed tools such as PCR cloning and sequencing, expression … and other important techniques crucial for all PCR methods. … Students and researchers interested in learning latest methods in PCR will find this book extremely useful. … This book presents cutting-edge methodologies crucial for all PCR methods. Given the rapid advances in the field of PCR methods, this third edition is essential and justifies replacement of the previous edition.” (Omer Iqbal, Doody’s Book Reviews, February, 2012)

“The book consists of 5 parts and 24 chapters. … Third edition of ‘PCR Prools’ represents very useful handbook written in form of original and modified PCR prools. Since it provides suggestions for solution of basic research as well as clinical and diagnostic problems, it will probably find its place in many molecular biologylaboratories.” (Ľubica Lacinová, General Physiology and Biochemistry, May, 2011)

From the Publisher