Placenta and Trophoblast: Methods and Protocols, Volume I

by Michael J. Soares, Joan S. Hunt

Overview

A collection of cutting-edge laboratory techniques for the study of trophoblast and placental biology. The techniques presented range from experimental animal models, to animal and human placental organ and cell culture systems, to morphological, biochemical, and molecular strategies for assessing trophoblast/placental growth, differentiation and function. Volume 1 provides readily reproducible prools for studying embryo-uterine implantation, trophoblast cell development, and the organization and molecular characterization of the placenta. Highlights include strategies for the isolation and culture of trophoblast cells from primates, ruminants, and rodents, and precise guidance to the molecular and cellular analysis of the placental phenotype. A companion second volume concentrates on methods for investigating placental function.

Product Details

ISBN-13:	9781627038133
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/20/2014
Series:	Methods in Molecular Medicine , #121
Edition description:	2006
Pages:	470
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Part I. Introduction

Placenta and Trophoblast: Methods and Prools: Overview I
Michael J. Soares and Joan S. Hunt

Part II. Methods for Studying Embryo Implantation and Uterine Biology

Methodologies to Study Implantation in Mice
Kaushik Deb, Jeff Reese, and Bibhash C. Paria

Blasyst Culture
D. Randall Armant

Isolation of Hormone Responsive Uterine Stromal Cells: An In Vitro Model for Stromal Cell Proliferation and Differentiation
Virginia Rider

Rat Decidual Cell Cultures
Yan Gu and Geula Gibori

The Immortalization of Human Endometrial Cells
Graciela Krikun, Gil Mor, and Charles Lockwood

Sheep Uterine Gland Knockout (UGKO) Model
Thomas E. Spencer and C. Allison Gray

A Baboon Model for Inducing Endometriosis
Asgerally T. Fazleabas

A Baboon Model for Simulating Pregnancy
Asgerally T. Fazleabas

The Common Marmoset Monkey as a Model for Implantation and Early Pregnancy Research
Almuth Einspanier, Kai Lieder, Ralf Einspanier, and Bettina Husen

Part III. In Vitro Trophoblast and Placental Model Systems

Mouse Trophoblast Stem Cells
Jennifer Quinn, Tilo Kunath, and Janet Rossant

Connexins and Trophoblast Cell Lineage Development
Mark Kibschull and Elke Winterhager

Rcho-1 Trophoblast Stem Cells: A Model System for Studying Trophoblast Cell Differentiation
Namita Sahgal, Lindsey N. Canham, Brent Canham, and Michael J. Soares

Bovine Trophoblast Cell Culture Systems: A Technique to Culture Bovine Trophoblast Cells Without Feeder Cells
Kazuyoshi Hashizume, Arata Shimada, Haruo Nakano, and Toru Takahashi

In Vitro Induction of Trophoblast from Human Embryonic Stem Cells
Ren-He Xu

Isolation and Culture of Term Human Trophoblast Cells
Margaret G. Petroff, Teresa A. Phillips, Hakhyun Ka, Judith L. Pace, andJoan S. Hunt

Production of Human Trophoblast Cell Lines
Guy St J. Whitley

Culture and Transfection of Human Choriocarcinoma Cells
Michael W. Wolfe

In Vitro Methods for Studying Vascularization of the Murine Allantois and Allantoic Union with the Chorion
Karen M. Downs

Part IV. Phenotypic Analysis of the Placenta

Phenotypic Analysis of the Mouse Placenta
David R. C. Natale, Maja Starovic, and James C. Cross

Phenotypic Analysis of the Rat Placenta
Rupasri Ain, Toshihiro Konno, Lindsey N. Canham, and Michael J. Soares

Analysis of the Structure of the Ruminant Placenta: Methods of Fixation, Embedding, and Antibody Localization at Light and Electron Microscope Levels
F. B. P. Wooding

Characterization of the Bovine Placenta by Cytoskeleton, Integrin Receptors, and Extracellular Matrix
Christiane D. Pfarrer

Molecular Markers for Human Placental Investigation
Berthold Huppertz

Correlative Microscopy of Ultrathin Cryosections in Placental Research
Toshihiro Takizawa and John M. Robinson

Vascular Corrosion Casting of the Uteroplacental and Fetoplacental Vasculature in Mice
Kathie J. Whiteley, Christiane D. Pfarrer, and S. Lee Adamson

Analysis of Fetal and Maternal Microvasculature in Ruminant Placentomes by Corrosion Casting
Rudolf Leiser and Christiane D. Pfarrer

Part V. Molecular Analysis and Gene Transfer Techniques

Microarray Analysis of Trophoblast Cells
Vikram Budhraja and Yoel Sadovsky

Gene Expression Microarray Data Analysis of Decidual and Placental Cell Differentiation
Sue Kong, Bruce J. Aronow, and Stuart Handwerger

Assays to Determine Allelic Usage of Gene Expression in the Placenta
Paul B. Vrana

Adenoviral-Mediated Gene Delivery to Trophoblast Cells
Bing Jiang and Carole R. Mendelson

Index

Editorial Reviews

Reviewer: Bruce A. Fenderson, PhD (Thomas Jefferson University)
Description: The mammalian embryo leaves its free-floating aquatic life shortly after fertilization and embeds itself in the uterine wall. Uterine endometrial tissue provides a necessary substrate for further development, and the developing placenta begins to regulate maternal-fetal exchange of blood gasses, nutrients, and immunological factors. This book introduces a wide array of modern laboratory protocols for studying the biology of the placenta. This is an exciting area of research with important implications for maternal health and fetal development. The book includes 31 chapters that provide detailed methods for studying the placenta in a variety of mammalian species. Examples of interesting research topics in Volume I of this two-book set include immortalization of endometrial cells, induction of trophoblast from embryonic stem cells, propagation of trophoblast stem cells, and microarray analysis of trophoblast cells. The principal focus of each chapter is on cell biology, biochemistry, and molecular biology.
Purpose: This book is part of the popular Methods in Molecular Medicine series, which provides detailed research methods, as well as tips and trouble-shooting advice. According to the editors, the aim of these two volumes is to offer contemporary approaches for studying the biology of the placenta. Volume I addresses protocols regarding mechanisms of implantation and placental development. Volume II continues with protocols for studying functions of the placenta, including regulation of maternal immune response and cellular adaptations to disease.
Audience: It is written for basic science and clinical researchers in mammalian embryology. Graduate students, medical students, research fellows, and established investigators will enjoy reading this book to broaden their horizons and gain insights into useful approaches for studying the origins, fate and functions of extra-embryonic tissues. Educators will find this book useful for illustrating state-of-the-art methods for studying embryogenesis. Biologists will appreciate the cross-species comparisons and multidisciplinary approach. The authors are experts in the field.
Features: This compact book comes with a companion CD that contains color illustrations. Each chapter includes a brief summary, list of key words, introduction, and complete listing of references. The line drawings, illustrations, photomicrographs are well done. Notes appended to each chapter enable the authors to provide explanations, tips, and suggestions for conducting successful experiments. Data generated from the authors' laboratories are used to illustrate key techniques.
Assessment: This book provides step-by-step instructions for conducting research in mammalian embryology. The contributions are well-written and user friendly. The methods and references are up-to-date. The chapters provide helpful introductions, reviews of methodology, and summaries of key experimental findings. The theoretical and practical importance of this research, and the diversity of topics presented, will help this book capture the attention of a wide audience of readers. The techniques are time-tested and so the book will provide an excellent laboratory resource for years to come. One suggestion I have for the authors of chapters in protocol books is that they should summarize the key questions under investigation. Too often, the basis for interest in a particular topic is assumed without explanation. This is an important contribution to the literature, and I recommend it for all those interested in learning more about maternal-fetal interactions during pregnancy.

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