Planarian Regeneration: Methods and Protocols

by Jochen C. Rink (Editor)

Overview

This volume explores the various facets of planaria as a biomedical model system and discusses techniques used to study the fascinating biology of these animals. The chapters in this book are divided into two parts: Part One looks at the biodiversity of planarian species, the molecular orchestration of regeneration, ecology of planarians in their natural habitats and their history as lab models. Part Two talks about experimental prools for studying planarians, ranging from the establishment of a planarian research colony, to RNA and DNA extraction techniques, all the way to single stem cell transplantations or metabolomics analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Planarian Regeneration: Methods and Prools is a valuable resource for both newcomers to the field and experts within established planarian laboratories.

Product Details

ISBN-13:	9781493978007
Publisher:	Springer New York
Publication date:	06/19/2018
Series:	Methods in Molecular Biology , #1774
Edition description:	1st ed. 2018
Pages:	573
Product dimensions:	7.01(w) x 10.00(h) x (d)

About the Author

Jochen Rink, PhD
Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstrasse 108, 01307 Dresden, Germany
Tel: [phone]
Email: [e-mail]

Table of Contents

Planarian Diversity and Phylogeny.- Stem Cells, Patterning, and Regeneration in Planarians-Self-Organization at the Organismal Scale.- The Ecology of Freshwater Planarians.- Planarians and the History of Animal Regeneration: Paradigm Shifts and Key Concepts in Biology.- Culturing Planarians in the Laboratory.- Total RNA Isolation from Planarian Tissues.- Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians.- Planarian High Molecular Weight DNA Isolation by Spooling.- Basic Histological Techniques for Planarians.- Fixation, Processing, and Immunofluorescent Labeling of Whole Mount Planarians.- Immunohishemistry on Paraffin-Embedded Planarian Tissue Sections.- Whole-Mount In Situ Hybridization of Planarians.- RNA In Situ Hybridization on Planarian Paraffin Sections.- General Principles of Planarian Embryogenesis and Its Analysis by In Situ Hybridization and Immunohishemistry Methods.- Whole-Mount BrdU Staining with Fluorescence In Situ Hybridization in Planarians.- Detection of Apoptotic Cells in Planarians by Whole-Mount TUNEL.- Systemic RNA Interference in Planarians by Feeding of dsRNA Containing Bacteria.- RNA Interference in Planarians: Feeding and Injection of Synthetic dsRNA.- A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells.- Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and Single-Cell Transplantation.- Tissue Transplantations in Planarians.- Live Imaging Planaria.- Processing Schmidtea mediterranea for Transmission Electron Microscopy: Classical and Microwave Techniques.- Tissue Extracts for Quantitative Mass Spectrometry of Planarian Proteins Using SILAC.- Quantification of Neurotransmitters from Intact and Regenerating Planarians Using UHPLC-MS/SRM Method.