Plant Senescence: Methods and Protocols

by Yongfeng Guo (Editor)

Overview

This detailed volume covers a wide variety of techniques either developed specially for plant senescence studies or optimized for studying senescing plants. After an introduction to the topic, the book continues with sections on phenotypic analysis and molecular markers of plant organ senescence, hormonal control of plant senescence, stress-induced senescence, molecular and cellular processes in plant senescence, as well as systems biology approaches. Written for the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.


Authoritative and practical, Plant Senescence: Methods and Prools aims to provide a useful hand book of standard prools for plant molecular biologists working on senescence.

Product Details

ISBN-13:	9781493976706
Publisher:	Springer New York
Publication date:	02/02/2018
Series:	Methods in Molecular Biology , #1744
Edition description:	1st ed. 2018
Pages:	367
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Concepts and Types of Senescence in Plants.- Initiation, Progression, and Genetic Manipulation of Leaf Senescence.- Phenotypic Analysis and Molecular Markers of Leaf Senescence.- Investigation of Petal Senescence by TRV-Mediated Virus-Induced Gene Silencing in Rose.- Phenotypic Analysis and Molecular Markers of Plant Nodule Senescence.- Quantitative Analysis of Floral Organ Abscission in Arabidopsis via a Petal Breakstrength Assay.- Characterization of Climacteric and Non-Climacteric Fruit Ripening.- Ethylene Treatment in Studying Leaf Senescence in Arabidopsis.- The Assay of Abscisic Acid-Induced Stomata Movement in Leaf Senescence.- The EPR Method for Detecting Nitric Oxide in Plant Senescence.- Hormone Treatments in Studying Leaf Senescence.- Methods to Study Darkness-Induced Leaf Senescence.- Salt Treatments and Induction of Senescence.- Methods for Elucidation of Plant Senescence in Response to C/N-Nutrient Balance.- Study of Cotton Leaf Senescence Induced by Alternaria alternata Infection.- Study of Hydrogen Peroxide as a Senescence-Inducing Signal.- Identification of Postharvest Senescence Regulators through Map-Based Cloning Using Detached Arabidopsis Inflorescences as a Model Tissue.- Chlorophyll and Chlorophyll Catabolite Analysis by HPLC.- Identification and Functional Analysis of Senescence-Associated Genes in Wheat.- Isolation, Purification, and Detection of MicroRNAs in Plant Senescence.- In Situ Detection of Programmed Cell Death in Senescing Nicotiana tabacum Leaves Using TUNEL Assay.- Activities of Vacuolar Cysteine Proteases in Plant Senescence.- Study of Autophagy in Plant Senescence.- Isolation of Chloroplasts for In Organelle Protein Degradation Assay.- Plant Cell Walls: Isolation and Monosaccharide Composition Analysis.- Visualizing Morphological Changes of Abscission Zone Cells in Arabidopsis by Scanning Electron Microscope.- RNA-Seq Analysis of the Transcriptome of Leaf Senescence in Tobacco.- Comprehensive Metabolomics Studies of Plant Developmental Senescence.