Plant Signal Transduction: Methods and Protocols

by Thomas Pfannschmidt (Editor)

Overview

During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Prools, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the prools for other plants. As part of the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory prools, and tips on trouble-shooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Plant Signal Transduction: Methods and Prools will benefit plant scientists wishing to improve their experimental approaches and delve further into this exciting and important field of study

Product Details

ISBN-13:	9781588299437
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/04/2008
Series:	Methods in Molecular Biology , #479
Edition description:	2009
Pages:	360
Product dimensions:	7.70(w) x 10.30(h) x 0.90(d)

Table of Contents

Multiple Dimensions in Plant Signal Transduction: An Overview.- Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions.- Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis.- Accurate Real-time Reverse Transcription Quantitative PCR.- Probing Spatio-temporal Intracellular Calcium Variations in Plants.- Dynamic Redox Measurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy.- Imaging of Reactive Oxygen Species In vivo.- Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single-Cysteine Mutant-Immobilized Resin.- Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes.- Examining Protein Stability and Its Relevance for Plant Growth and Development.- Probing Circadian Rhythms in Chlamydomonas reinhardtii by Functional Proteomics.- Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells.- Fluorescence Cross-Correlation Spectroscopy of Plant Proteins.- The Determination of Protein-protein Interactions by the Mating-based Split-ubiquitin system (mbSUS).- Functional Complementation of Yeast Mutants to Study Plant Signalling Pathways.- Phosphatase Activities Analyzed by in vivo Expressions.- Chromatin Immunoprecipitation Experiments to Investigate In Vivo Binding of Arabidopsis Transcription Factors to Target Sequences.- Fluorescence-based Electrophoretic Mobility Shift Assay in the Analysis of DNA-binding Proteins.- Analysis of Plant Regulatory DNA sequences by the Yeast-One-Hybrid Assay.- Analysis of Plant Regulatory DNA Sequences by Transient Protoplast Assays and Computer Aided Sequence Evaluation.- RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System.

Editorial Reviews

From the reviews:

"This text pertinently addresses the field of signal transduction in plants. … This volume covers a wide range of basic and cutting edge techniques that can be used in any number of areas of plant signaling research. … This volume … covers a wide range of cutting edge techniques that are leading to novel insight into signal transduction pathways in plant biology. … this is a fine reference text that surveys a range of current plant experimental tools and techniques." (Beronda L. Montgomery, Plant Science Bulletin, October, 2009)

From the Publisher