PRINS and In Situ PCR Protocols

PRINS and In Situ PCR Protocols

by Franck Pellestor (Editor)

Paperback(Softcover reprint of hardcover 2nd ed. 2006)

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Product Details

ISBN-13: 9781617376368
Publisher: Springer-Verlag New York, LLC
Publication date: 12/16/2010
Series: Methods in Molecular Biology , #334
Edition description: Softcover reprint of hardcover 2nd ed. 2006
Pages: 244
Product dimensions: 5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

Part I. Basic Methodology

New Rapid Multicolor PRINS Protocol
Ju Yan, Macoura Gadji, Kada Krabchi, and Régen Drouin

PRINS Combined With Peptide Nucleic Acid Labeling
Franck Pellestor and Petra Paulasova

PRINS Combined With Indirect Immunofluorescence
Philippe Coullin

PRINS for the Detection of Unique Sequences
Stephen S. Wachtel and Avirachan T. Tharapel

Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS)
Jørn Koch

Analysis of Sperm Aneuploidy by PRINS
Franck Pellestor, Brigitte Andréo, Jacques Puechberty, Geneviève Lefort, and Pierre Sarda

In Situ PCR Protocols
Omar Bagasra and Twaina Harris

Part II. Research and Clinical Applications

Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS
Jørn Koch

PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei
Antonella Russo

PRINS for the Detection of Gene Deletions in Cancer
Avirachan T. Tharapel and Stephen S. Wachtel

Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene
Liborio Stuppia, Dario La Sala, and Caterina Cinti

Brain Tissue Preparations for Chromosomal PRINS Labeling
Ivan Y. Iourov, Svetlana G. Vorsanova, Franck Pellestor, and Yuri B. Yurov

PRINS on Plant Chromosomes
Jirí Macas, Alice Navrátilová, Marie Kubaláková, and Jaroslav Dolezel

Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood
Kada Krabchi, Macoura Gadji, Ju Yan, and Régen Drouin

PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos
Franck Pellestor, Brigitte Andréo, Tal Anahory, and Samir Hamamah

Microdissection With PCR In Situ
Allen T. Christian and ChristineHara

Application of Reverse Transcription In Situ PCR in Cancer Analysis
Tamotsu Takeuchi, Yoshihiro Adachi, Hiroshi Sonobe, and Yuji Ohtsuki

In Situ Reverse Transcription PCR on Plant Tissues
Zbigniew Przybecki, Ewa Siedlecka, Marcin Filipecki, and Ewa Urbanczyk-Wochniak

In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection
Barry Slobedman

Development and Adaptation of the PRINS Technology: An Overview
Franck Pellestor

Latest Developments in In Situ PCR
Omar Bagasra and Twaina Harris


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