PRINS and In Situ PCR Protocols
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Prools edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful prools for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and prools for PRINS detection of unique sequences in situ.
1117338242
PRINS and In Situ PCR Protocols
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Prools edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful prools for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and prools for PRINS detection of unique sequences in situ.
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PRINS and In Situ PCR Protocols

PRINS and In Situ PCR Protocols

by Franck Pellestor (Editor)
PRINS and In Situ PCR Protocols

PRINS and In Situ PCR Protocols

by Franck Pellestor (Editor)

Overview

The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Prools edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful prools for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and prools for PRINS detection of unique sequences in situ.

Product Details

ISBN-13: 9781617376368
Publisher: Springer-Verlag New York, LLC
Publication date: 12/16/2010
Series: Methods in Molecular Biology , #334
Edition description: Softcover reprint of hardcover 2nd ed. 2006
Pages: 244
Product dimensions: 5.98(w) x 9.02(h) x 0.02(d)

Table of Contents

Basic Methodology.- New Rapid Multicolor PRINS Prool.- PRINS Combined With Peptide Nucleic Acid Labeling.- PRINS Combined With Indirect Immunofluorescence.- PRINS for the Detection of Unique Sequences.- Single-Copy and Point Mutation Analysis (Rolling-Circle PRINS).- Analysis of Sperm Aneuploidy by PRINS.- In Situ PCR Prools.- Research and Clinical Applications.- Detection and Sizing of Telomeric and Other Simple Repeats by Dideoxy-PRINS.- PRINS Evaluation of Chromosome Instability in Mammalian Cells by Detection of Repetitive DNA Sequences in Micronuclei.- PRINS for the Detection of Gene Deletions in Cancer.- Combined Fluorescence In Situ Hybridization and PRINS for the Analysis of the Dystrophin Gene.- Brain Tissue Preparations for Chromosomal PRINS Labeling.- PRINS on Plant Chromosomes.- Dual-Color PRINS for In Situ Detection of Fetal Cells in Maternal Blood.- PRINS as an Efficient Tool for Aneuploidy Assessment in Human Oocytes and Preimplantation Embryos.- Microdissection With PCR In Situ.- Application of Reverse Transcription In Situ PCR in Cancer Analysis.- In Situ Reverse Transcription PCR on Plant Tissues.- In Situ PCR for the Detection of Human Cytomegalovirus in Suspension Cells During the Latent Phase of Infection.- Development and Adaptation of the PRINS Technology.- Latest Developments in In Situ PCR.
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