Protein Design: Methods and Applications

by Valentin Kïhler (Editor)

Overview

Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical prools and instructive reviews to aid in the creation of new experiments.

Product Details

ISBN-13:	9781493914852
Publisher:	Springer New York
Publication date:	09/11/2014
Series:	Methods in Molecular Biology , #1216
Edition description:	2nd ed. 2014
Pages:	279
Product dimensions:	7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

De novo Design of Stable α-helices.- Design of Monomeric Water-soluble b-hairpin and β-sheet Peptides.- Combination of Theoretical and Experimental Approaches for the Design and Study of Fibril-forming Peptides.- Posttranslational Incorporation of Non-canonical Amino Acids in the RNase S System by Semisynthetic Protein Assembly.- Design, Synthesis, and Study of Fluorinated Proteins.- High-Quality Combinatorial Protein Libraries Using the Binary Patterning Approach.- Methods for Library-Scale Computational Protein Design.- Symmetric Protein Architecture in Protein Design: Top-Down Symmetric Deconstruction.- Identification of Protein Scaffolds for Enzyme Design using Scaffold Selection.- Computational Design of Novel Enzymes without Cofactors.- De novo Design of Peptide Scaffolds as Novel Pre-organised Ligands for Metal-ion Coordination.- Computational Design of Metalloproteins.- Incorporation of Modified and Artificial Cofactors into Naturally Occurring Protein Scaffolds.- Computational Redesign of Metalloenzymes for Catalyzing New Reactions.