Protein Hydrolysates in Biotechnology / Edition 1

by Vijai K. Pasupuleti, Arnold L. Demain

Overview

Protein hydrolysates, otherwise commonly known as peptones or peptides, are used in a wide variety of products in fermentation and biotechnology industries. The term “peptone” was first introduced in 1880 by Nagelli for growing bacterial cultures. However, later it was discovered that peptones derived from the partial digestion of proteins would furnish organic nitrogen in readily available form. Ever since, p- tones, which are commonly known as protein hydrolysates, have been used not only for growth of microbial cultures, but also as nitrogen source in commercial fermen- tions using animal cells and recombinant microorganisms for the production of value added products such as therapeutic proteins, hormones, vaccines, etc. Today, the characterization, screening and manufacturing of protein hyd- lysates has become more sophisticated, with the introduction of reliable analytical instrumentation, high throughput screening techniques coupled with statistical design approaches, novel enzymes and efficient downstream processing equipment. This has enabled the introduction of custom-built products for specialized appli- tions in diverse fields of fermentation and biotechnology, such as the following. 1. Protein hydrolysates are used as much more than a simple nitrogen source. For example, the productivities of several therapeutic drugs made by animal cells and recombinant microorganisms have been markedly increased by use of p- tein hydrolysates. This is extremely important when capacities are limited. 2. Protein hydrolysates are employed in the manufacturing of vaccines by ferm- tation processes and also used as vaccine stabilizers.

Product Details

ISBN-13:	9781402066733
Publisher:	Springer Netherlands
Publication date:	09/13/2010
Edition description:	2010
Pages:	229
Product dimensions:	6.10(w) x 9.25(h) x 0.02(d)

Table of Contents

1 Applications of Protein Hydrolysates in Biotechnology Vijai K. Pasupuleti Chris Holmes Arnold L. Demain 1

2 State of the Art Manufacturing of Protein Hydrolysates Vijai K. Pasupuleti Steven Braun 11

3 Towards an Understanding of How Protein Hydrolysates Stimulate More Efficient Biosynthesis in Cultured Cells André Siemensma James Babcock Chris Wilcox Hans Huttinga 33

4 Benefits and Limitations of Protein Hydrolysates as Components of Serum-Free Media for Animal Cell Culture Applications: Protein Hydrolysates in Serum Free Media Juliet Lobo-Alfonso Paul Price David Jayme 55

5 Oligopeptides as External Molecular Signals Affecting Growth and Death in Animal Cell Cultures František Franek 79

6 Use of Protein Hydrolysates in Industrial Starter Culture Fermentations Madhavi (Soni) Ummadi Mirjana Curic-Bawden 91

7 Protein Hydrolysates from Non-bovine and Plant Sources Replaces Tryptone in Microbiological Media Yamini Ranganathan Shifa Patel Vijai K. Pasupuleti R. Meganathan 115

8 The Use of Protein Hydrolysates for Weed Control Nick Christians Dianna Liu Jay Bryan Unruh 127

9 Physiological Importance and Mechanisms of Protein Hydrolysate Absorption Brian M. Zhanghi James C. Matthews 135

10 Protein Hydrolysates/Peptides in Animal Nutrition Jeff McCalla Terry Waugh Eric Lohry 179

11 Protein Hydrolysates as Hypoallergenic, Flavors and Palatants for Companion Animals Tilak W. Nagodawithana Lynn Nelles Nayan B. Trivedi 191

12 The Development of Novel Recombinant Human Gelatins as Replacements for Animal-Derived Gelatin in Pharmaceutical Applications David Olsen Robert Chang Kim E. Williams James W. Polarek 209

Index 227