Protein Structure, Stability, and Folding

by Kenneth P. Murphy (Editor)

Overview

In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.

Product Details

ISBN-13:	9780896036826
Publisher:	Springer-Verlag New York, LLC
Publication date:	04/01/2001
Series:	Methods in Molecular Biology , #168
Edition description:	2001
Pages:	252
Product dimensions:	6.10(w) x 9.25(h) x 0.02(d)

Table of Contents

Stabilization of Protein Structure.- Protein Stabilization by Naturally Occurring Osmolytes.- TheThermodynamic Linkage Between Protein Structure, Stability, and Function.- Measuring the Conformational Stability of a Protein by Hydrogen Exchange.- Modeling the Native State Ensemble.- Conformational Entropy in Protein Folding.- Turn Scanning.- Laser Temperature-Jump Methods for Studying Folding Dynamics.- Kinetics of Conformational Fluctuations by EX1 Hydrogen Exchange in Native Proteins.- Molecular Dynamics Simulations of Protein Unfolding/Folding.

Editorial Reviews

Ten papers describe some of the newest experimental and theoretical methods for investigating protein structure, stability, and folding. Among the techniques discussed are the many methods for calculating protein stability dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches include the use of co-solvents, applications of hydrogen exchange techniques, temperature-jump methods, and mutagenesis experiments. The theory behind each experimental approach is emphasized. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

"Both theoretical and experimental approaches for studying protein structure, stability, and folding are described in the book. The fundamental concepts are clearly presented....The topics on modeling and simulation would be useful for protein designers....The book starts with a concise overview on the fundamental thermodynamic concepts and governing forces for protein folding." - PHARMACEUTICAL RESEARCH

"focuses on the structure-stability relationship and in the well-balanced mixture of experimental and theoretical chapters, all of which are written by experts in the fields." - Chembiochem

From the Publisher