Protein Structure: A Practical Approach / Edition 2

by T. E. Creighton

Overview

Protein Structure: A Practical Approach gives general methods for determining protein structure that will be very widely applicable, from the simple electrophoretic, chromatographic, and spectrophotometric techniques to the relatively more complex and sophisticated methods of mass spectrometry and ultracentrifugation. This second edition has been fully updated to embrace the most recent developments in the field, and will be invaluable to all involved in protein research.

Product Details

ISBN-13:	9780199636181
Publisher:	Oxford University Press
Publication date:	05/08/1997
Series:	Practical Approach Series , #174
Edition description:	REV
Pages:	406
Product dimensions:	9.20(w) x 6.10(h) x 1.10(d)

About the Author

Creighton, T. E. (European Molecular Biology Laboratory, Heidelberg)

The contributors represent the specialties of biochemistry and molecular biology. Most are from universities and research institutes in the U.S., the U.K., France, and Germany. Institutions prominently represented include Institut Pasteur, European Molecular Biology Laboratory-Heidelberg, Univ of Utah, and Brandeis Univ.

Table of Contents

1. Protein molecular weight determination by sodium dodecyl sulfate polyacrylamide gel electrophoresis2. Protein analysis by mass spectrometry3. Immunological detection of proteins of known sequence4. Identification of common post-translational modifications5. Peptide mapping6. Counting integral numbers of residues by chemical modification7. Disulfide bonds between cysteine residues8. Analysis of protein conformation by gel electrophoresis9. Hydrodynamic properties of proteins10. How to determine the molar absorbance coefficient of a protein11. Optical spectroscopy to characterize protein confirmation and conformational changes12. Measuring the conformational stability of a protein13. Immunochemical analysis of protein conformation14. Stabilization of protein structure by solvents

Editorial Reviews

Reviewer: Gene A. Homandberg, PhD (Rush Medical College of Rush University)
Description: This second edition manual of methods in protein structure studies includes, but is not limited to, chapters on mass determination by gel analysis and mass spectrometry, immunologic detection of proteins of known sequence, identification of post-translational modifications, peptide mapping, determination of disulfide bonding patterns, analysis of protein conformation by gel electrophoresis, analysis of hydrodynamic properties, use of spectroscopy to characterize protein conformation and conformational changes, immunologic analysis of protein conformation, and stabilization of protein by solvents.
Purpose: This manual is meant to update common methods given in the first edition as well as introduce newer chapters on mass spectrometry and ultracentrifugation, while still retaining the original emphasis on simple and common methods.
Audience: The audience should include not only student researchers, since it will be of great value in experimental design, but also experienced protein chemists who need an update on methods already in use. Also, molecular biologists with less experience in these methods should find these protocols easy to use and very practical.
Features: The features of the manual include ample and relevant diagrams, protocol boxes with easy-to-read detailed instructions, and concisely written general descriptions. A list of suppliers is also included.
Assessment: This manual is highly recommended for anyone using protein structure targeted methods. It will be highly useful to anyone, regardless of their experience, because the methods are updated and are presented very clearly. The diverse, relevant, and important topics covered also add to the value of this book.

Doody's Review Service

4 Stars! from Doody

This second edition manual of methods in protein structure studies includes, but is not limited to, chapters on mass determination by gel analysis and mass spectrometry, immunologic detection of proteins of known sequence, identification of post-translational modifications, peptide mapping, determination of disulfide bonding patterns, analysis of protein conformation by gel electrophoresis, analysis of hydrodynamic properties, use of spectroscopy to characterize protein conformation and conformational changes, immunologic analysis of protein conformation, and stabilization of protein by solvents. This manual is meant to update common methods given in the first edition as well as introduce newer chapters on mass spectrometry and ultracentrifugation, while still retaining the original emphasis on simple and common methods. The audience should include not only student researchers, since it will be of great value in experimental design, but also experienced protein chemists who need an update on methods already in use. Also, molecular biologists with less experience in these methods should find these protocols easy to use and very practical. The features of the manual include ample and relevant diagrams, protocol boxes with easy-to-read detailed instructions, and concisely written general descriptions. A list of suppliers is also included. This manual is highly recommended for anyone using protein structure targeted methods. It will be highly useful to anyone, regardless of their experience, because the methods are updated and are presented very clearly. The diverse, relevant, and important topics covered also add to the value of this book.

Gene A. Homandberg