The Annual Beltsville Symposium serves as a forum for presenting recent developments in basic research that contribute solutions to agricultural problems. The eleventh in this series focuses on instrumentation tech niques which provide powerful new research tools. These tools will provide information that can lead to a better understanding of biological and physical processes critical to the solution of today's agricultural problems. The tools discussed include isotope technology, separation techniques, microscopy, electro-magnetic spectroscopy, resonance and mass spectrometry and microcomputers. This Symposium brought together scientists who are at the forefront of the development of many of these tools and those who are applying them to problems directly related to agricultural research. WALDEMAR KLASSEN, Director Beltsville Area VII CONTRIBUTORS AND THEIR AFFILIATIONS Norman G. Anderson Youhanna Fares Proteus Technologies, Inc. Phytokinetics Inc. Rockville, MD 20852 College Station, TX 77840 U.S.A. U.S.A. Cherie L. Fisk Edwin D. Becker Office of Research Services Office of Research Services NIH NIH Bethesda, MD 20892 Bethesda, MD 20892 U.S.A. U.S.A. Klaus Biemann John D. Goeschl Phytokinetics, Ltd. Department of Chemistry College Station, TX 77840 Massachusetts Institute of Technology Cambridge, MA 02139 U.S.A. U.S.A. R.J. Griesbach Florist and Nursery Crops Laboratory B.A. Bolton Development Quality Laboratory Horticultural Science Institute Agricultural Research Service USDA Western Regional Research Center Beltsville, MD 20705 Agricultural Research Service USDA U.S.A. Albany, CA 94710 U.S.A.
Table of ContentsIsotope Technology.- 1 Recent Advances in Detectors for Radioisotopes.- 2 Short-Lived Isotopic Kinetics: a Window to the Inside.- 3 Application of Stable Isotopes in Analysis and Metabolism.- 4 Flow Cytometry and Sorting of Cells and Organelles.- 5 Field Flow Fractionation of Biological Materials.- 6 Research Instrumentation for the 21st Century: Progress Toward Complete Genomic Maps and Sequence Data Bases, and Indexes of Protein Gene Products.- Microscopy.- 7 Curent Developments and Future Trends in Electron Microscopy.- 8 Electron Probe X-Ray Microanalysis and Digital Elemental X-Ray Imaging and Their Application to Biomedical Histological Sections and Whole Cells.- 9 Applications of Microspectrophgtometry.- Electro-Magnetic Spectroscopy.- 10 Satellite Instrumentation for Monitoring Agricultural Conditions.- 11 Near-Infrared Used in Process Chemistry.- 12 Fourier Transform Infrared Spectrometry: a Tool for Modern Agricultural Research.- 13 Application of Raman Spectroscopy to Molecular Agricultural Research.- Resonance Spectrometry.- 14 NMR: New Techniques for Chemical Analysis and Biological Investigation.- 15 Agricultural/Biological Applications of NMR.- 16 Applications of Electron Paramagnetic Resonance Spectroscopy to the Study of Bio-Membranes.- 17 Recent Developments in Mass Spectrometry.- 18 Recent Developments in High Mass and Tandem Mass Spectrometry.- 19 Prospective Trends in Quantitative Mass Spectrometry.- Microprocessors in Data Reduction and Presentation.- 20 Microcomputer Interfacing in the Analytical Laboratory.- 21 Computer Aided Interpretation of Spectra.- 22 Computed Tomography: the Use and Interpretation of Imaged Data in Animal Research.- 23 Interactive Microprocessor Graphics as Aid in Physiological Modeling: Two Examples.