Retinal Degeneration: Methods and Protocols
Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Prools, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Retinal Degeneration: Methods and Prools aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.

1110843206
Retinal Degeneration: Methods and Protocols
Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Prools, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Retinal Degeneration: Methods and Prools aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.

159.0 In Stock
Retinal Degeneration: Methods and Protocols

Retinal Degeneration: Methods and Protocols

Retinal Degeneration: Methods and Protocols

Retinal Degeneration: Methods and Protocols

Paperback(Softcover reprint of the original 1st ed. 2013)

$159.00 
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Overview

Over the past decades, knowledge about the cellular and molecular basis underlying the visual process has remarkably increased. In Retinal Degeneration: Methods and Prools, expert researchers in the field provide a guide of relevant and state-of-the-art methods for studying retinal homeostasis and disease. These include methods and techniques for addressing cell culture systems and animal models of disease, their generation, their phenotypic and molecular characterization as well as their use in therapeutic approaches to the retina. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Retinal Degeneration: Methods and Prools aids scientists in continuing to study the cutting-edge techniques of retinal cell biology in health and disease.


Product Details

ISBN-13: 9781493959693
Publisher: Springer-Verlag New York, LLC
Publication date: 08/23/2016
Series: Methods in Molecular Biology , #935
Edition description: Softcover reprint of the original 1st ed. 2013
Pages: 384
Product dimensions: 7.01(w) x 10.00(h) x (d)

Table of Contents

Identification and Analysis of Inherited Retinal Disease Genes.-Mouse Models for Studies of Retinal Degeneration and Diseases.-Retinal Fundus Imaging in Mouse Models of Retinal Diseases.-Functional Phenotyping of Mouse Models with ERG.-Phenotyping of Mouse Models with OCT.-Light Damage as a Model of Retinal Degeneration.-N-Methyl-D-Aspartate (NMDA)-mediated Excitotoxic Damage – A Mouse Model of Acute Retinal Ganglion Cell Damage.-Generation of Transgenic X. laevis Models of Retinal Degeneration.-Analysis of Photoreceptor Degeneration in the Zebrafish Danio rerio.-Analysis of Optokinetic Response in Zebrafish by Computer Based Eye Tracking.-Analysis of the Drosophila Compound Eye with Light and Electron Microscopy.-Cell Specific Markers for the Identification of Retinal Cells by Immunofluorescence Microscopy.-A Method of Horizontally-Sliced Preparation of the Retina.-Detection of DNA Fragmentation in Retinal Apoptosis by TUNEL.-High-throughput RNA in situ Hybridization in Mouse Retina.- Assessment of Mihondrial Damage in Retinal Cells and Tissues using Quantitative Polymerase Chain Reaction for Mihondrial DNA Damage and Extracellular Flux Assay for Mihondrial Respiration Activity.-Analysis of Photoreceptor Rod Outer Segment Phagocytosis by RPE Cells in situ.-Ca2+ Microfluorimetry in Retinal Müller Glial Cells.-Functional Analysis of Retinal Microglia and their Effects on Progenitors.-Analysis of Photoreceptor Outer Segment Phagocytosis by RPE Cells in Culture.-Ca2+ Imaging Techniques to Analyze Ca2+ Signaling in Cells and to Monitor Neuronal Activity in the Retina.-Double Chromatin Immunoprecipitation:Analysis of Target Co-Occupancy of Retinal Transcription Factors.-Quantifying the Activity of cis-regulatory Elements in the Mouse Retina by Explant Electroporation.-Optimized Technique for Subretinal Injections in Mice.-Adeno Associated Viral Vectors for Gene Therapy of Inherited Retinal Degenerations.-Barrier Modulation in Drug Delivery to the Retina.

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