Sertoli Cells: Methods and Protocols

by Marco G. Alves (Editor), Pedro F. Oliveira (Editor)

Overview

This detailed volume explores the use of Sertoli cells as models in male reproductive biology or as supporters for other cell types, and it answers the need for standardized prools for their in vitro culture. Prepared for non-specialists as well as experienced researchers that may be interested in a multidisciplinary approach to study these cells, the book examines these remarkable cells that present some unique features that go far beyond the physical and nutritional support of spermatogenesis. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Sertoli Cells: Methods and Prools serves as a valuable resource for experienced researchers in reproductive biology, particularly andrologists, but also for future investigators and young students starting to study these extraordinary cells.

Product Details

ISBN-13:	9781493976973
Publisher:	Springer New York
Publication date:	02/16/2018
Series:	Methods in Molecular Biology , #1748
Edition description:	1st ed. 2018
Pages:	297
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Establishment of Primary Culture of Sertoli Cells.- Evaluation of the Purity of Sertoli Cell Primary Cultures.- Preparation of Testicular Samples for Histology and Immunohishemistry.- Rabbit Sertoli Cells: Immunohishemical Profile from Neonatal to Adult Age.- Identification of Proliferative and Apoptotic Sertoli Cells Using Fluorescence and Confocal Microscopy.- Sertoli Cells Preparation for Co-Immunoprecipitation.- Profiling Signaling Proteins in Sertoli Cells by Co-Immunoprecipitation.- Phagocytosis by Sertoli Cells: Analysis of Main Phagocytosis Steps by Confocal and Electron Microscopy.- A Method for In Vivo Induction and Ultrastructural Detection of Mitophagy in Sertoli Cells.- Assessing Autophagy in Sertoli Cells.- Molecular Mechanisms and Signaling Pathways Involved in the Nutritional Support of Spermatogenesis by Sertoli Cells.- Assessing Sertoli Cells Metabolic Activity.- Proteome Profiling of Sertoli Cells Using a GeLC-MS/MS Strategy.- Gene Silencing of Human Sertoli Cells Utilizing Small Interfering RNAs.- Testicular Cell Selective Ablation Using Diphtheria Toxin Receptor Transgenic Mice.- Regulation of Blood-Testis Barrier (BTB) Dynamics, Role of the Actin, and Microtubule-Based Cytoskeletons.- Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay.- Computational Methods Involved in Evaluating the Toxicity of the Reproductive Roxicants in Sertoli Cell.- A Stopped-Flow Light Scattering Methodology for Assessing the Osmotic Water Permeability of Whole Sertoli Cells.- Cryopreservation of Human Testicular Tissue by Isopropyl-Controlled Slow Freezing.

Editorial Reviews

The pictures illustrating the book are all of high quality and the readers will be satisfied and certainly not disappointed while running through the chapters: exactly in the same manner you cannot stop of eating chapters or cherries, one is calling the other!” (CarloAlberto Redi, European Journal of Hishemistry, Vol. 63 (01), 2019)​

From the Publisher