Single-Molecule Studies of Nucleic Acids and Their Proteins
This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.
1133670146
Single-Molecule Studies of Nucleic Acids and Their Proteins
This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.
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Single-Molecule Studies of Nucleic Acids and Their Proteins

Single-Molecule Studies of Nucleic Acids and Their Proteins

Single-Molecule Studies of Nucleic Acids and Their Proteins

Single-Molecule Studies of Nucleic Acids and Their Proteins

Overview

This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

Product Details

ISBN-13: 9780198530923
Publisher: Oxford University Press
Publication date: 02/20/2019
Series: Oxford Graduate Texts
Pages: 240
Product dimensions: 9.80(w) x 6.90(h) x 0.70(d)

About the Author

David Bensimon, Research Director and Professor, LPS-ENS, PSL, CNRS and Department of Chemistry and Biochemistry, UCLA, USA, Vincent Croquette, Research Director, LPS-ENS, PSL, CNRS, France,Jean-Francois Allemand, Professor, LPS-ENS, PSL, CNRS, France,Xavier Michalet, Researcher, Department of Chemistry and Biochemistry, UCLA, USA,Terence Strick, Research Director and Principle Investigator, IBENS, PSL, CNRS, France

David Bensimon and Vincent Croquette have been co-leaders of a group at the LPS-ENS that has been studying single DNA molecules and their interactions with proteins since 1991. Jean-Francois Allemand, Terence Strick and Xavier Michalet have been their students and collaborators ever since. They have jointly developed magnetic traps and have been studying various aspects of single molecule DNA/protein transactions. Since joining the lab of Professor S. Weiss at UCLA , Xavier Michalet has been focusing on the use of fluorescent and spectroscopic methods to study single biomolecules.

Table of Contents

1. Introduction to DNA2. Manipulating DNA3. Fluorescence Spectroscopy and Microscopy Techniques4. The Mechanical Properties of Nucleic Acids5. Structural Transitions in DNA6. Single Molecule Studies of DNA-associated Motors7. DNA and RNA Polymerases8. DNA Helicases9. Topoisomerases10. Conclusions
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