Small Molecule Microarrays: Methods and Protocols

by Mahesh Uttamchandani (Editor), Shao Q. Yao (Editor)

Overview

Small molecule microarrays (SMM) were introduced just a decade ago in 1999 and, within a short space of time, have already established themselves as a vibrant, next generation platform for high-throughput screening. Small Molecule Microarrays: Methods and Prools showcases a collection of contributions guiding researchers toward ways in which small molecule microarray technology may be deployed for multiplexed screening and profiling. Organized by the categories of small molecules presented on the microarrays, this detailed volume describes in-depth techniques for chemical libraries, peptide libraries, and carbohydrate microarrays. Written in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible prools, vital tips on troubleshooting this often difficult technology, and advice on avoiding known pitfalls. Authoritative and cutting-edge, Small Molecule Microarrays: Methods and Prools provides meticulous depictions of key hands-on experience and seeks to inspire a future generation of microarray practitioners to take this significant technology forward.

Product Details

ISBN-13:	9781493958153
Publisher:	Springer-Verlag New York, LLC
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #669
Edition description:	Softcover reprint of the original 1st ed. 2010
Pages:	228
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

The Expanding World of Small Molecule Microarrays.- Construction of Photo-Cross-Linked Microarrays of Small Molecules.- Small Molecule Microarray: Functional-Group Specific Immobilization of Small Molecules.- Detecting Protein–Small Molecule Interactions Using Fluorous Small-Molecule Microarrays.- A Method for Small Molecule Microarray-Based Screening for the Rapid Discovery of Affinity-Based Probes.- Nanodroplet Chemical Microarrays and Label-Free Assays.- Nanodroplet Microarrays for High-Throughput Enzyme Screening.- The Application of the Chemical Array for Biological Study.- Peptide Arrays with a Chip.- In Situ Chemical Modification of Peptide Microarrays: Characterization by Desorption/Ionization on Silicon Nanowires.- In Situ Chemical Modification of Peptide Microarrays: Application to the Study of the Antibody Responses to Methylated Antigens.- Peptide Microarrays on Coated Silicon Slides for Highly Sensitive Antibody Detection.- Antibody Signatures Defined by High-Content Peptide Microarray Analysis.- High Density Peptide Microarrays for Proteome-Wide Fingerprinting of Kinase Activities in Cell Lysates.- A Peptide Microarray for Detecting Protein Kinase Activity in Cell Lysates.- Chemical Microarrays Constructed by Selective Attachment of Hydrazide-Conjugated Substances to Epoxide Surfaces and Their Applications.- A Microarray-Based Method to Perform Nucleic Acid Selections.