Somatic Stem Cells: Methods and Protocols

by Shree Ram Singh (Editor)

Overview

Stem cells are found in almost all organisms from the early stages of development to the end of life. There are several types of stem cells and all of them may prove useful for medical research; however, each of the different types has both promise and limitations. Somatic Stem Cells: Methods and Prools presents selected genetic, molecular, and cellular techniques used in somatic stem cell research and its clinical application. Chapters focus on the isolation, characterization, purity, plasticity, and clinical uses of somatic stem cells from a variety of human and animal tissues. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Through and intuitive, Somatic Stem Cells: Methods and Prools seeks to provides scientists with the fundamental techniques of stem cell research and its potential application in regenerative medicine.

Product Details

ISBN-13:	9781617798146
Publisher:	Springer-Verlag New York, LLC
Publication date:	05/23/2012
Series:	Methods in Molecular Biology , #879
Edition description:	2012
Pages:	546
Product dimensions:	7.01(w) x 10.00(h) x 0.06(d)

Table of Contents

Current Thoughts on the Therapeutic Potential of Stem Cell.-A Unique FACS Method to Isolate Stem Cells in Planarian.-Identification of Neural Stem Cells in the Drosophila Larval Brain.-Generation and Staining of Intestinal Stem Cell Lineage in Adult Midgut.-Developing a Quantitative In Vivo Tissue Reconstitution Assay to Assess the Relative Potency of Candidate Populations of Mouse Oesophageal Epithelial Cells.-Identification, Isolation, and Culture of Intestinal Epithelial Stem Cells from Murine Intestine.-Isolation and Characterization of Distal Lung Progenitor Cells.-Transplantation of Mouse Fetal Liver Cells for Analyzing the Function of Hematopoietic Stem and Progenitor Cells.-Convenient and Efficient Enrichment of the CD133+ Liver Cells From Rat Fetal Liver as a Source of Liver Stem/Progenitor Cells.-Assessing the Potential Clinical Utility of Transplantations of Neural and Mesenchymal Stem Cells for Treating Neurodegenerative Diseases.-Functional Identification of Neural Stem Cell-Derived Oligodendrocytes.-Stem/Progenitor Cells in Murine Mammary Gland: Isolation and Functional Characterization.-A Reporter Assay to Detect Transfer and Targeting of miRNAs in Stem Cell-Breast Cancer Co-Cultures.-Isolation, Culture and Osteogenic/chondrogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cells.-Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue.-Collection, Processing and Banking of Umbilical Cord Blood Stem Cells for Transplantation and Regenerative Medicine.-Generation of Functional Islets from Human Umbilical Cord and Placenta Derived Mesenchymal Stem Cells.-Isolation and Characterization of Human Prostate Stem/Progenitor Cells.-Isolation and Expansion of Adult Cardiac Stem/Progenitor Cells in the Form of Cardiospheres from Human Cardiac Biopsies and Murine Hearts.-Isolation and Differentiation of Human Cardiomyocyte Progenitor Cells Into Cardiomyocytes.-Isolation, Expansion andCharacterization of Human Islet-Derived Progenitor Cells.-Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli.-Endothelial Colony Forming Progenitor Cell Isolation and Expansion.-Isolation and Characterization of Stem Cell-Enriched Human and Canine Hair Follicle Keratinocytes.-Human Salivary Gland Stem Cells: Isolation, Propagation and Characterization.-Identification, Isolation, Characterization and Banking of Human Dental Pulp Stem Cells.-Isolation and Differentiation Potential of Fibroblast-Like Stromal Cells Derived from Human Skin.-Immortalization of Human Mesenchymal Stromal Cells with Telomerase and Red Fluorescence Protein Expression.-Genetic Modification of Mesenchymal Stem Cells.-Methodology, Biology and Clinical Applications of Human Mesenchymal Stem Cells.-In Vitro Production of Enucleated Red Blood Cells from Hematopoietic Stem and Progenitor Cells.-Methods for Cancer Stem Cell Detection and Isolation.-Biocompatible Nanoparticle Labelling of Stem Cells and their Distribution in Brain.