Subcellular Fractionation: A Practical Approach / Edition 1

by John Graham, David Rickwood

Overview

Many investigations into the structure and function of cells and tissues require the isolation of a particular membrane or subcellular component (organelle). This book covers all the necessary aspects, from the homogenization of cells via a variety of separation techniques, to the isolation and characterization of peroxisomes and other organelles. Basic separation techniques needed in all cell biology laboratories are given and presented in the context of a number of related topics such as the labelling of ligands, the kinetics of their internalization and analysis of data, and the electron microscopy of organelles. Subcellular Fractionation contains expert advice and detailed methodology, and will be invaluable to researchers in this important area.

Product Details

ISBN-13:	9780199634941
Publisher:	Oxford University Press
Publication date:	03/27/1997
Series:	Practical Approach Series , #173
Edition description:	New Edition
Pages:	360
Product dimensions:	6.12(w) x 9.12(h) x 0.76(d)

About the Author

Graham, John M. (John Moores Univ, Liverpool); Rickwood, David (Univ of Essex)

The contributors represent the specialties of cell biology, biochemistry, biology, and molecular genetics. Most are from universities and research institutes in the U.K., Germany, and Norway. Institutions prominently represented include Univ of Oslo, Max-Planck-Institut, Univ of Glasgow, and Ruprecht-Karls Universitat Heidelberg.

Table of Contents

1. Homogenization of tissues and cells2. Isolation of subcellular fragments3. Isolation and characterization of nuclei and nuclear subfractions4. Subcellular fractionation of mitochondria5. Isolation and characterization of peroxisomes6. Lysosomes and endocytosis7. The membranes of the secretory and exocytic pathways8. Isolation and purification of functionally intact mitochondria and chloroplasts from plant cells9. Ribosomes and polysomes10. Electron microscopy of organelles

Editorial Reviews

4 Stars! from Doody

This is a techniques book discussing many of the principles and practices of the subcellular fractionation of bacterial, plant, and animal cells. It is a recent addition to the Practical Approach Series, an extensive series published by the IRL Press of Oxford University Press. The objectives of this volume are to provide a practical guide to techniques of subcellular fractionation. This is an important aspect of modern biochemical and cell biological research, as it has been for more than 40 years. These objectives are met admirably. The techniques are intended for anyone actually carrying out any of the procedures described in the text. As such, the book is very well written. Each of the 10 chapters is written by one or more individuals who are clearly experts on the specific topic. The authors are affiliated with one of several major European universities. Each chapter has numerous step-by-step protocols for the techniques described therein. An appendix gives the names and address for suppliers of many of the pieces of equipment and apparatus described in the chapters. Illustrations (diagrams, micrographs, photographs) are not a centerpiece for this book. Those that are included are of good, but not exceptional, quality. The references are thorough and current. The book has a plain but attractive appearance. This book should be a very useful reference for anyone who relies on cell fractionation techniques. Life science libraries certainly have a copy of this book on their shelves. Individuals who use these techniques on a variety of tissues and organs may wish to own a copy of this book.

Alvin Telser

Reviewer: Alvin Telser, PhD (Northwestern University Feinberg School of Medicine)
Description: This is a techniques book discussing many of the principles and practices of the subcellular fractionation of bacterial, plant, and animal cells. It is a recent addition to the Practical Approach Series, an extensive series published by the IRL Press of Oxford University Press.
Purpose: The objectives of this volume are to provide a practical guide to techniques of subcellular fractionation. This is an important aspect of modern biochemical and cell biological research, as it has been for more than 40 years. These objectives are met admirably.
Audience: The techniques are intended for anyone actually carrying out any of the procedures described in the text. As such, the book is very well written. Each of the 10 chapters is written by one or more individuals who are clearly experts on the specific topic. The authors are affiliated with one of several major European universities.
Features: Each chapter has numerous step-by-step protocols for the techniques described therein. An appendix gives the names and address for suppliers of many of the pieces of equipment and apparatus described in the chapters. Illustrations (diagrams, micrographs, photographs) are not a centerpiece for this book. Those that are included are of good, but not exceptional, quality. The references are thorough and current. The book has a plain but attractive appearance.
Assessment: This book should be a very useful reference for anyone who relies on cell fractionation techniques. Life science libraries certainly have a copy of this book on their shelves. Individuals who use these techniques on a variety of tissues and organs may wish to own a copy of this book.

Doody's Review Service