Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids

'Techniques of Lipidology' can be considered a lipid research classic. This, the 3rd edition, describes how to extract lipids, to separate lipid classes from a total lipid extract, to analyze lipids by TLC, and to hydrolyze lipids. The book also includes a wealth of IR, NMR and mass spectrometry data about lipids and their hydrolysis products.

Earlier editions of this book have served as a practical and fundamental guide to Lipidology for thousands of scientists worldwide. The original work by Dr. Morris Kates was published in 1972 and was followed by a second edition in 1986. In this, the 3rd edition, Dr. Kates incorporates key aspects of his own and related research since 1986, adding detail and expanding content to include groundbreaking findings related to cardiolipin, extremophiles, and the composition of cell membranes.

The overall format and organization of the third edition is similar to that of the second edition, but has been considerably extended in scope and coverage. Comprehensive lists of figures, tables and equations have been added for the convenience of the reader. Finally, the book has been typeset on pages that are 35% larger than those of earlier editions. We trust that this revised edition will be useful to a wide group of experienced researchers and scientists in the fields of molecular biology, biochemistry and medicine.

Material covered - highlights:

  • Classification of lipids
  • Spectral characteristics of lipids
  • Reactions and calculations
  • Equipment and supplies
  • 160+ Concise procedures:
    • Lipid extraction and analysis
    • Separation of lipid mixtures
    • Radioisotopic labelling, extraction and analysis of lipids
    • Identification of lipids and lipid moieties

For ease of reference, the book now includes lists of figures, tables, and equations, and an expanded index and table of contents.

35 tables
43 figures
113 equations
350+ structures
800+ references
160+ concise procedures

Audience: Lipid researchers, as well as biochemists and molecular biologists whose research has led them to the analytical biochemistry of lipids.

1114314424
Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids

'Techniques of Lipidology' can be considered a lipid research classic. This, the 3rd edition, describes how to extract lipids, to separate lipid classes from a total lipid extract, to analyze lipids by TLC, and to hydrolyze lipids. The book also includes a wealth of IR, NMR and mass spectrometry data about lipids and their hydrolysis products.

Earlier editions of this book have served as a practical and fundamental guide to Lipidology for thousands of scientists worldwide. The original work by Dr. Morris Kates was published in 1972 and was followed by a second edition in 1986. In this, the 3rd edition, Dr. Kates incorporates key aspects of his own and related research since 1986, adding detail and expanding content to include groundbreaking findings related to cardiolipin, extremophiles, and the composition of cell membranes.

The overall format and organization of the third edition is similar to that of the second edition, but has been considerably extended in scope and coverage. Comprehensive lists of figures, tables and equations have been added for the convenience of the reader. Finally, the book has been typeset on pages that are 35% larger than those of earlier editions. We trust that this revised edition will be useful to a wide group of experienced researchers and scientists in the fields of molecular biology, biochemistry and medicine.

Material covered - highlights:

  • Classification of lipids
  • Spectral characteristics of lipids
  • Reactions and calculations
  • Equipment and supplies
  • 160+ Concise procedures:
    • Lipid extraction and analysis
    • Separation of lipid mixtures
    • Radioisotopic labelling, extraction and analysis of lipids
    • Identification of lipids and lipid moieties

For ease of reference, the book now includes lists of figures, tables, and equations, and an expanded index and table of contents.

35 tables
43 figures
113 equations
350+ structures
800+ references
160+ concise procedures

Audience: Lipid researchers, as well as biochemists and molecular biologists whose research has led them to the analytical biochemistry of lipids.

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Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids

Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids

by Morris Kates
Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids

Techniques of Lipidology: Isolation, Analysis, and Identification of Lipids

by Morris Kates

Hardcover(3rd Revised ed.)

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Overview

'Techniques of Lipidology' can be considered a lipid research classic. This, the 3rd edition, describes how to extract lipids, to separate lipid classes from a total lipid extract, to analyze lipids by TLC, and to hydrolyze lipids. The book also includes a wealth of IR, NMR and mass spectrometry data about lipids and their hydrolysis products.

Earlier editions of this book have served as a practical and fundamental guide to Lipidology for thousands of scientists worldwide. The original work by Dr. Morris Kates was published in 1972 and was followed by a second edition in 1986. In this, the 3rd edition, Dr. Kates incorporates key aspects of his own and related research since 1986, adding detail and expanding content to include groundbreaking findings related to cardiolipin, extremophiles, and the composition of cell membranes.

The overall format and organization of the third edition is similar to that of the second edition, but has been considerably extended in scope and coverage. Comprehensive lists of figures, tables and equations have been added for the convenience of the reader. Finally, the book has been typeset on pages that are 35% larger than those of earlier editions. We trust that this revised edition will be useful to a wide group of experienced researchers and scientists in the fields of molecular biology, biochemistry and medicine.

Material covered - highlights:

  • Classification of lipids
  • Spectral characteristics of lipids
  • Reactions and calculations
  • Equipment and supplies
  • 160+ Concise procedures:
    • Lipid extraction and analysis
    • Separation of lipid mixtures
    • Radioisotopic labelling, extraction and analysis of lipids
    • Identification of lipids and lipid moieties

For ease of reference, the book now includes lists of figures, tables, and equations, and an expanded index and table of contents.

35 tables
43 figures
113 equations
350+ structures
800+ references
160+ concise procedures

Audience: Lipid researchers, as well as biochemists and molecular biologists whose research has led them to the analytical biochemistry of lipids.


Product Details

ISBN-13: 9780981375700
Publisher: Newport Somerville
Publication date: 09/03/2010
Edition description: 3rd Revised ed.
Pages: 452
Product dimensions: 6.14(w) x 9.21(h) x 1.00(d)

About the Author

Dr. Morris Kates 1923 - 2013 Morris Kates graduated with a degree in Physics and Chemistry in 1945 and obtained his Ph.D. in 1948 from the University of Toronto. His Ph.D. thesis described the first synthesis of L-a-lecithins and related compounds, from which followed the systematic study of lipid-water systems. After receiving his Ph.D. he joined the National Research Council in Ottawa where he worked from 1951 to 1968. During his early years there, he established the oxindole nature of the alkaloid gelsemine. This, and his many other accomplishments established him as an internationally prominent researcher in lipid biochemistry. In 1968 Dr. Kates moved to the University of Ottawa where he taught organic chemistry and biochemistry. He served as vice-dean of the Faculty of Science and Engineering from 1975 to 1979 and as chairman of the Department of Biochemistry from 1982 to 1985. The breadth of Dr. Kates' research spanned structure and synthesis, metabolism, enzymology, and physical chemistry of lipids. Over forty graduate students and postdoctoral fellows benefitted from Dr. Kates' mentorship, and with his encouragement, developed their careers in Canada and internationally. In the course of his career, over a dozen visiting professors came to Ottawa, Canada from various countries to work with Dr. Kates. Dr. Kates was president of the Canadian Biochemical Society, and was co-editor of the Canadian Journal of Biochemistry for 11 years from 1973 to 1984. He also served on committees of the Canadian Medical Research Council (MRC). He was invited to become a Fellow of the Royal Society of Canada in 1973, and was awarded the Society's 'Excellence in Research' award in 1981. In 1984, Dr Kates was the honoured recipient of the American Oil Chemists' Society Supelco Research Award. His achievements were further recognized in 1994 when he was honoured with the UK Biochemical Society's Morton Lectureship. As an emeritus professor he maintained an active interest in the analysis, structure, determination, biosynthesis and function of membrane phospholipids, biodiesel production, and the nutritional value of lipids. From 2005 until 2010 Dr. Kates dedicated himself to writing the third edition of Techniques of Lipidology. Earlier editions of this book have served as a laboratory guide to thousands of scientists worldwide. In addition to his love of science, Dr. Kates was also a prolific composer and an avid supporter of the Canadian Music Centre for many decades.

Table of Contents

Abbreviated Table of Contents

Preface
Acknowledgements
Health, Safety and Environmental Precautions
List of Tables
List of Figures
List of Equations
List of Abbreviations

Chapter 1. Definition and Classification of Lipids
1.1. Hydrocarbons
1.1.1 ...
1.2. Alcohols
1.2.1 ...
1.3. Long-chain amino alcohols
1.4. Aldehydes
1.4.1 ...
1.5. Ketones and quinones
1.5.1 ...
1.6. Fatty acids
1.6.1 ...
1.7. Waxes
1.7.1 ...
1.8. Acyl esters of sterols and vitamin alcohols
1.8.1 ...
1.9. Acyl esters of polyhydric alcohols
1.9.1 ...
1.10. Ethers of polyhydric alcohols
1.10.1 ...
1.11. Phospholipids
1.11.1 ...
1.12. Glycolipids
1.12.1 ...
1.13. Sulfur-containing lipids
1.13.1 ...
1.14. Amino acid-containing lipids
1.14.1 ...

Chapter 2. Materials and Equipment
2.1. Solvents
2.2. Reference compounds
2.3. Glassware
2.3.1 ...
2.4. Evaporators
2.4.1 ...
2.5. Stirrers and heaters
2.6. Analytical Instruments
2.6.1 ...

Chapter 3. Lipid Extraction Procedures
3.1. General precautions
3.2. General lipid extraction procedure
3.2.1 ...
3.3. Storage of lipid extracts
3.4. Final purification and preparation of lipids for analysis
3.4.1 ...

Chapter 4. General Analytical Procedures
4.1. Dry weight
4.2. Phosphorus
4.2.1 ...
4.3. Nitrogen
4.3.1 ...
4.4. Ester determination
4.5. Total sugars
4.6. Iodine value
4.7. Cholesterol
4.7.1 ...
4.8. Total fatty acids and unsaponifiable material
4.8.1 ...
4.9. Neutralization equivalent (titration of acidic lipids)
4.10. Plasmalogens
4.10.1 ...
4.11. N-Acyl containing lipids (sphingolipids)
4.12. Water-soluble constituents
4.12.1 ...
4.13. Vicinal-glycol determination
4.13.1 ...
4.14. Glycerol ether or diol ether constituents
4.14.1 ...
4.15. Sulfur
4.15.1 ...
4.16. Spectrometric analyses
4.16.1 ...

Chapter 5. Separation of Lipid Mixtures
5.1. Solvent fractionation
5.1.1 ...
5.2. Column chromatography (liquid chromatography, LC)
5.2.1 ...
5.3. Thin-layer chromatography (TLC)
5.3.1 ...
5.4. Gas-liquid chromatography (GLC or GC)
5.4.1 ...
5.5. Gel permeation chromatography (GPC)
5.5.1 ...
5.6. Matrix assisted laser desorption / ionization (MALDI) mass spectrometry

Chapter 6. Radioisotopic Techniques in Lipidology
6.1. Radioactive precursors
6.1.1 ...
6.2. Radio-labelling of cellular lipid components
6.2.1 ...
6.3. Fractionation and isolation of radioisotopically labeled lipid components
6.3.1 ...
6.5. Autoradiography and scanning of chromatograms
6.5.1 ...

Chapter 7. Identification of Individual Lipids and Lipid Moieties
7.1. Neutral lipids
7.1.1 ...
7.2. Complex polar lipids
7.2.1 ...
7.3. Glycolipids
7.3.1 ...

References
Subject Index
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