Table of Contents
Establishing and Maintaining an IVF Laboratory
Setting up an ART laboratory; Jacques Cohen, Mina Alikani, Antonia Gilligan, and Tim Schimmel
Quality control: Maintaining stability in the laboratory; David H. McCulloh
The ART laboratory: Current standards; Cecilia Sjöblom
Gamete Collection, Preparation and Selection
Evaluation of sperm; Kaylen M. Silverberg and Tom Turner
Sperm preparation techniques; Harold Bourne, Janell Archer, David H. Edgar, and H. W. Gordon Baker
Sperm chromatin assessment; Ashok Agarwal, Igor Tsarev, Juris Erenpreiss, and Rakesh Sharma
Oocyte retrieval and selection; Laura F. Rienzi and Filippo M. Ubaldi
Preparation and evaluation of oocytes for ICSI; Irit Granot and Nava Dekel
Hyaluronic acid binding-mediated sperm selection for ICSI; Gabor Huszar
Intracytoplasmic morphologically selected sperm injection; Monica Antinori
Use of in vitro maturation in a clinical setting: Patient populations and outcomes; Yoshiharu Morimoto, Aisaku Fukuda, and Manabu Satoh
Micromanipulation
Micromanipulation; Frank L. Barnes
Intracytoplasmic sperm injection: Technical aspects; Queenie V. Neri, Devin Monahan, Zev Rosenwaks, and Gianpiero D. Palermo
Assisted hatching; Anna Veiga and Itziar Belil
Human embryo biopsy procedures; Alan R. Thornhill, Christian Ottolini, and Alan H. Handyside
Culture, Selection and Transfer of the Human Embryo
Analysis of fertilization; Thomas Ebner
Culture systems for the human embryo; David K. Gardner and Michelle Lane
Evaluation of embryo quality: Analysis of morphology and quantification of nutrient utilization and the metabolome; Denny Sakkas and David K. Gardner
Evaluation of embryo quality: Time-lapse imaging to assess embryo morphokinesis; Natalia Basile, Juan García-Velasco, and Marcos Meseguer
Evaluation of embryo quality: Proteomic strategies; Mandy Katz-Jaffe
Cryopreservation
The human oocyte: Controlled rate cooling; Andrea Borini and Veronica Bianchi
The human oocyte: Vitrification; Masashige Kuwayama
The human embryo: Slow freezing; Nikica Zaninovic, Richard Bodine, Robert N. Clarke, Sam Jones, Ye Zhen, and Lucinda L. Veeck Gosden
The human embryo: Vitrification; Zsolt Peter Nagy, Ching-Chien Chang, and Gábor Vajta
Diagnosis of Genetic Disease in Preimplantation Embryos
Severe male factor: Genetic consequences and recommendations for genetic testing; Willy Lissens and Katrien Stouffs; Polar body biopsy and its clinical application; Markus Montag
Preimplantation genetic diagnosis for infertility; Dagan Wells and Elpida Fragouli
Genetic analysis of the embryo; Yuval Yaron, Veronica Gold, Sagit Peleg-Schalka, and Mira Malcov
Implantation
The analysis of endometrial receptivity; Tamara Garrido-Gomez, Francisco Domínguez, Maria Ruiz, Felip Vilella, and Carlos Simon
Future Directions and Clinical Applications
Human embryonic stem cells; Rachel Eiges, Michal Avitzour, and Benjamin Reubinoff
Microfluidics in ART: Current progress and future directions; Jason E. Swain, Thomas B. Pool, Shuichi Takayama, and Gary D. Smith
Index