Tick-Borne Viruses: Methods and Protocols

by Saravanan Thangamani (Editor)

Overview

This volume covers a variety of the latest scientific methods utilized in tick-borne virus (TBV) research. The chapters in this book explore detailed prools on how to conduct in-depth research on TBVs; enhance understanding of the virus’ biology; transmission mechanisms; and the creation of effective countermeasures. Following the established Methods in Molecular Biology series format, each chapter introduces the topic, lists necessary materials and reagents, offers step-by-step reproducible laboratory prools, and includes troubleshooting tips to help avoid common issues.

Cutting-edge and thorough, Tick-Borne Viruses: Methods and Prools is a comprehensive and innovative resource for researchers, clinicians, and public health experts interested in exploring the complexities of TBVs.

Product Details

ISBN-13:	9781071645864
Publisher:	Springer US
Publication date:	06/14/2025
Series:	Methods in Molecular Biology , #2936
Pages:	152
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Tick Cell Culture Methods.- Virus Isolation and Culture from Field Collected Ticks: In Vitro.- Virus Isolation from Field-Collected Ticks: In Vivo.- Generation of Ticks Infected with Tick-Borne Encephalitis Virus.- Generation of Virus Infected Ticks: Synchronous Infection and Feeding on Viremic Animals.- Human Skin Ex-Vivo Culture for Tick-Borne Virus Infection Studies.- Colorado Tick Fever Virus: Quantification, Propagation, Storage, and Detection.- Isolation and Quantitation of Dabie bandavirus.- Immunofluorescence Assays to Visualize Tick-Borne Flaviviruses in Mouse Skin or Brain.- Immunohishemical Assays to Visualize Tick-Borne Flaviviruses at the Tick Feeding Site.- RNA In-Situ Hybridization to Visualize Tick-Borne Viruses.- Quantification of Tick-Borne Flaviviruses Using Focus Forming Assays.- Quantification of Neutralizing Antibodies to Tick-Borne Viruses using Focus Neutralization Assays.- Methods for Evaluation of Antivirals and Neutralizing Antibodies Against Tick-Borne Encephalitis Virus.- Exploring Tick-Borne Encephalitis Virus-Host Cell Interactions using Electron Tomography: Methodologies and Prools.