Transcription Factor Protocols
The effort to sequence the human genome is now moving toward a c- clusion. As all of the protein coding sequences are described, an increasing emphasis will be placed on understanding gene function and regulation. One important aspect of this analysis is the study of how transcription factors re- late transcriptional initiation by RNA polymerase II, which is responsible for transcribing nuclear genes encoding messenger RNAs. The initiation of Class II transcription is dependent upon transcription factors binding to DNA e- ments that include the core or basal promoter elements, proximal promoter elements, and distal enhancer elements. General initiation factors are involved in positioning RNA polymerase II on the core promoter, but the complex - teraction of these proteins and transcriptional activators binding to DNA e- ments outside the core promoter regulate the rate of transcriptional initiation. This initiation process appears to be a crucial step in the modulation of mRNA levels in response to developmental and environmental signals. Transcription Factor Prools provides step-by-step procedures for key techniques that have been developed to study DNA sequences and the protein factors that regulate the transcription of protein encoding genes. This volume is aimed at providing researchers in the field with the well-detailed prools that have been the hallmark of previous volumes of the Methods in Molecular ™ Biology series.
1117338191
Transcription Factor Protocols
The effort to sequence the human genome is now moving toward a c- clusion. As all of the protein coding sequences are described, an increasing emphasis will be placed on understanding gene function and regulation. One important aspect of this analysis is the study of how transcription factors re- late transcriptional initiation by RNA polymerase II, which is responsible for transcribing nuclear genes encoding messenger RNAs. The initiation of Class II transcription is dependent upon transcription factors binding to DNA e- ments that include the core or basal promoter elements, proximal promoter elements, and distal enhancer elements. General initiation factors are involved in positioning RNA polymerase II on the core promoter, but the complex - teraction of these proteins and transcriptional activators binding to DNA e- ments outside the core promoter regulate the rate of transcriptional initiation. This initiation process appears to be a crucial step in the modulation of mRNA levels in response to developmental and environmental signals. Transcription Factor Prools provides step-by-step procedures for key techniques that have been developed to study DNA sequences and the protein factors that regulate the transcription of protein encoding genes. This volume is aimed at providing researchers in the field with the well-detailed prools that have been the hallmark of previous volumes of the Methods in Molecular ™ Biology series.
109.99
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Transcription Factor Protocols
306
Transcription Factor Protocols
306Paperback(Softcover reprint of hardcover 1st ed. 2000)
$109.99
109.99
In Stock
Product Details
| ISBN-13: | 9781617370816 |
|---|---|
| Publisher: | Springer-Verlag New York, LLC |
| Publication date: | 11/10/2010 |
| Series: | Methods in Molecular Biology , #130 |
| Edition description: | Softcover reprint of hardcover 1st ed. 2000 |
| Pages: | 306 |
| Product dimensions: | 5.98(w) x 9.02(h) x 0.03(d) |
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