Transcription Factor Protocols

Transcription Factor Protocols

by Martin J. Tymms (Editor)

Paperback(Softcover reprint of hardcover 1st ed. 2000)

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Product Details

ISBN-13: 9781617370816
Publisher: Springer-Verlag New York, LLC
Publication date: 11/10/2010
Series: Methods in Molecular Biology , #130
Edition description: Softcover reprint of hardcover 1st ed. 2000
Pages: 306
Product dimensions: 5.98(w) x 9.02(h) x 0.03(d)

Table of Contents

Isolation of Target Gene Promoter/Enhancer Sequences by Whole Genome PCR Method
Dennis K. Watson, Richard Kitching, Calvin Vary, Ismail Kola, and Arun Seth

In Vivo Footprinting Using UV Light and Ligation-Mediated PCR
Gerd P. Pfeifer and Stella Tommasi

Identification of DNaseI Hypersensitive Sites Within Nuclei
Peter N. Cockerill

Analysis of In Vivo Methylation
Hans-Henrik M. Dahl and Wendy M. Hutchison

Detection of Transcription Factor Partners with a Yeast One Hybrid Screen
Michael Sieweke

Inverse PCR (IPCR) for Obtaining Promoter Sequence
Tony Triglia

PCR-Directed Linker Scanning Mutagenesis
Kurt Gustin and Robert D. Burk

Transfection Technologies
Elaine T. Schenborn

The Use of Particle-Mediated Gene Transfer for the Study of Promoter Activity in Somatic Tissues
Charles M. Nicolet and Ning-Sun Yang

Optimizing Electroporation Conditions for the Transformation of Mammalian Cells
William C. Heiser

Calcium Phosphate Transfection of Mammalian Cultured Cells
Elaine T. Schenborn and Virginia Goiffon

DEAE-Dextran Transfection of Mammalian Cultured Cells
Elaine T. Schenborn and Virginia Goiffon

Liposome-Mediated Transfection of Mammalian Cells
Elaine T. Schenborn and Jennifer Oler

Assays for Transcriptional Activity Based on the Luciferase Reporter Gene
S. Roy Himes and M. Frances Shannon

Transient Transfection of Schneider Cells in the Study of Transcription Factors
Guntram Suske

Triplex-Forming Oligonucleotides and Their Use in the Analysis of Gene Transcription
Marina Kochetkova and M. Frances Shannon

Expression and Purification of Histidine-Tagged Transcription Factors
Donna R. Cohen

Generation of Transcription Factors in Rabbit Reticulocyte Lysate Depleted of Endogenous DNA-Binding Protein
Ulrich Kruse, Thorsten T. Ebel, and Albrecht E. Sippel

Electrophoretic Mobility Shift Assays
Peter L. Molloy

In Vitro Promoter Analysis Using Nuclear Extracts and G-Free Cassette Vectors
Martin J. Tymms

In Vitro Transcription Using Competitor Oligonucleotides to Deplete Specific Transcription Factors
Fujiko Watt

Computer Software of Eukaryotic Promoter Analysis
Dan S. Prestridge


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