This book provides guidance and easy to follow protocols for the appli cation of molecular biological techniques for researchers and ophthalm ologists with no training in molecular biology. It describes the best applications for the discussed techniques and provides detailed troubl e shooting. In addition, the influence of these emerging new technolog ies on everyday clinical services is also discussed in detail. The boo k is recommended to all scientists, clinicians and optometrist working in eye research with scientific questions answerable by molecular bio logical technology.
Table of Contents
Basic Molecular Biology Techniques, Chooi-May Lai. Transient Transfection of Human Retinoblastoma Cells: Application to the Analysis of the Regulatory Regions of Photoreceptor-Specific Genes, Debora B. Farber, Leonid E. Lerner, and Andrea S. Viczian. In Situ Hybridization, Xian-Jie Yang. Immunocyhemical Studies of the Retina, Ann H. Milam. Antisense DNA Technology, Piroska Elizabeth Rakoczy. Inhibition of Gene Expression by Ribozymes, William W. Hauswirth, Lynn C. Shaw, Patrick O. Whalen, Jason J. Fritz, D. Alan White, and Alfred S. Lewin. Intraocular Delivery of Recombinant Virus, Fong-Qi Liang, Vibha Anand, Albert M. Maguire, and Jean Bennett. Preparation of Recombinant Adenoviruses, Karl G. Csaky. Generation of Recombinant Adeno-Associated Virus, Fabienne Rolling. Preparation of Recombinant Retroviruses, Xian-Jie Yang. Generation of Transgenic Mice for Studies of Ocular Development and Disease, Abha R. Gupta, Nadine S. Dejneka, Albert M. Maguire, and Jean Bennett. Generation of Knockout Animal Models, T. Michael Redmond. Mutation Analysis of Ocular Genes, Michael Danciger. Ophthalmologists' Perspective, Susan M. Downes, N. H. Victor Chong, and Alan C. Bird. Index.