Pub. Date:
Springer-Verlag New York, LLC
Xenopus Protocols: Cell Biology and Signal Transduction / Edition 1

Xenopus Protocols: Cell Biology and Signal Transduction / Edition 1

by X. Johnï Liu
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Product Details

ISBN-13: 9781617375200
Publisher: Springer-Verlag New York, LLC
Publication date: 11/19/2010
Series: Methods in Molecular Biology , #322
Edition description: Softcover reprint of hardcover 1st ed. 2006
Pages: 489
Product dimensions: 5.98(w) x 9.02(h) x 0.04(d)

Table of Contents

Resources for Genetic and Genomic Studies of Xenopus
Steven L. Klein, Daniela S. Gerhard, Lukas Wagner, Paul Richardson, Lynn M. Schriml, Amy K. Sater, Wesley C. Warren, and John D. McPherson

The Physiology of the Xenopus laevis Ovary
Melissa A. Rasar and Stephen R. Hammes

Oocyte Isolation and Enucleation
X. Shawn Liu and X. Johné Liu

Xenopus tropicalis Oocytes: More Than Just a Beautiful Genome
Jean-François L. Bodart and Nicholas S. Duesbery

Oocyte Expression With Injection of Purified T7 RNA Polymerase
Xavier Altafaj, Nathalie Joux, Michel Ronjat, and Michel De Waard

Visualization of the Cytoskeleton in Xenopus Oocytes and Eggs by Confocal Immunofluorescence Microscopy
Bret E. Becker and David L. Gard

Multiphoton Laser Scanning Microscopy as a Tool for Xenopus Oocyte Research
Angela M. Prouty, Jun Wu, Da-Ting Lin, Patricia Camacho, and James D. Lechleiter

Imaging Ca2+ Signals in Xenopus Oocytes
Sheila L. Dargan, Angelo Demuro, and Ian Parker

Chromosomal DNA Replication in a Soluble Cell-Free System Derived From Xenopus Eggs
Antonin V. Tutter and Johannes C. Walter

Chromatin Assembly of DNA Templates Microinjected Into Xenopus Oocytes
Danièle Roche, Geneviève Almouzni, and Jean-Pierre Quivy

Pre-mRNA Splicing in the Nuclei of Xenopus Oocytes
Kyong Hwa Moon, Xinliang Zhao, and Yi-Tao Yu

Chromatin Immunoprecipitation for Studying Transcriptional Regulation in Xenopus Oocytes and Tadpoles
David Stewart, Akihiro Tomita, Yun-Bo Shi, and Jiemin Wong

Cytoplasmic mRNA Polyadenylation and Translation Assays
Maria Piqué, José Manuel López, and Raúl Méndez

Xenopus Egg Extracts: A Model System to Study Proprotein Convertases
Kathleen I. J. Shennan

Analysis of Molecular Chaperones Using a Xenopus Oocyte Protein Refolding Assay
John J. Heikkila,Angelo Kaldis, and Rashid Abdulle

Ubiquitin-Mediated Protein Degradation in Xenopus Egg Extracts
Anna Castro, Suzanne Vigneron, Cyril Bernis, Jean-Claude Labbé, and Thierry Lorca

Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms
Reiner Peters

Use of Xenopus laevis Oocyte Nuclei and Nuclear Envelopes in Nucleocytoplasmic Transport Studies
Reiner Peters

Nuclear Pore Complex Structure and Plasticity Revealed by Electron and Atomic Force Microscopy
Bohumil Maco, Birthe Fahrenkrog, Ning-Ping Huang, and Ueli Aebi

In Vitro Study of Nuclear Assembly and Nuclear Import Using Xenopus Egg Extracts
Rene C. Chan and Douglass J. Forbes

Use of Intact Xenopus Oocytes in Nucleocytoplasmic Transport Studies
Nelly Panté

Studying the Mechanosensitivity of Voltage-Gated Channels Using Oocyte Patches
Catherine E. Morris, Peter F. Juranka, Wei Lin, Terence J. Morris, and Ulrike Laitko

Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides
Steven David Buckingham, Luanda Pym, and David Barry Sattelle

Microtransplantation of Neurotransmitter Receptors From Cells to Xenopus Oocyte Membranes: New Procedure for Ion Channel Studies
Ricardo Miledi, Eleonora Palma, and Fabrizio Eusebi

Reconstitution of Golgi Disassembly by Mitotic Xenopus Egg Extracts in Semi-Intact MDCK Cells
Fumi Kano, Katsuya Takenaka, and Masayuki Murata

Exploring RNA Virus Replication in Xenopus Oocytes
Andrea V. Gamarnik and Raul Andino

Study of Apoptosis in Vitro Using the Xenopus Egg Extract Reconstitution System
Paula Deming and Sally Kornbluth

Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomics
Ken-ichi Sato, Ken-ichi Yoshino, Alexander A. Tokmakov, Tetsushi Iwasaki, Kazuyoshi Yonezawa, and Yasuo

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