Yeast Protocols / Edition 2

by Wei Xiao

Overview

In this second edition of a widely used classic laboratory manual, leading experts utilize the tremendous progress and technological advances that have occurred to create a completely new collection of not only the major basic techniques, but also advanced prools for yeast research and for using yeast as a host to study genes from other organisms. The authors provide detailed methods for the isolation of subcellular components-including organelles and macromolecules, for the basic cellular and molecular analysis specific for yeast cells, and for the creation of conditional mutant phenotypes that lend themselves to powerful genome manipulation. Additional prools offer advanced approaches to study genetic interactions, DNA and chromatin metabolism, gene expression, as well as the foreign genes and gene products in yeast cells.

Product Details

ISBN-13:	9781617375699
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/19/2010
Series:	Methods in Molecular Biology , #313
Edition description:	Second Edition 2006
Pages:	392
Product dimensions:	5.98(w) x 9.02(h) x 0.35(d)

Table of Contents

Basic Investigations in Saccharomyces cerevisiae.- Isolation of Nucleic Acids.- Purification of Yeast Peroxisomes.- Isolation of Yeast Plasma Membranes.- Isolation of Yeast Mihondria.- Extraction of Yeast Lipids.- Two-Dimensional Gel Electrophoresis of Total Yeast Proteins.- Pulsed-Field Gel Electrophoresis of Budding Yeast Chromosomes.- Analysis of Yeast Lipids.- Yeast Fluorescence Microscopy.- Intracellular Expression of Recombinant Antibody Fluorescent Protein Fusions for Localization of Target Antigens in Schizosaccharomyces pombe.- Yeast Transformation by the LiAc/SS Carrier DNA/PEG Method.- Mutagenesis.- Gene Disruption in the Budding Yeast Saccharomyces cerevisiae.- Inducible Degron and Its Application to Creating Conditional Mutants.- Synthetic Lethal Screen.- Synthetic Genetic Array Analysis in Saccharomyces cerevisiae.- Two-Dimensional Agarose Gel Analysis of DNA Replication Intermediates.- Chromatin Assembly in a Crude Fraction From Yeast Cells.- Chromatin Immunoprecipitation to Study Protein-DNA Interactions in Budding Yeast.- Isolation of Yeast Nuclei and Micrococcal Nuclease Mapping of Nucleosome Positioning.- Study of Transcriptional Regulation Using a Reporter Gene Assay.- Assessing Telomeric Phenotypes.- Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast.- Production of Heterologous Proteins in Yeast With the Aid of the Hsp150? Carrier.- Yeast Two-Hybrid System Screening.- Import of Precursor Proteins Into Isolated Yeast Mihondria.

Editorial Reviews

From Reviews of the First Edition...

"...will benefit the apprentices...and the experienced scientist venturing into a new technique or changing yeast species...should join the other 'cookery' books on the laboratory shelf." -Journal of Emergency Medicine

"...a useful addition to life sciences libraries and to laboratories embarking on studies with yeast"

-Quarterly Review of Biology

From the Publisher