Zygotic Genome Activation: Methods and Protocols

by Matthew C. Good (Editor)

Overview

This volume details cutting-edge techniques used to gain new and deeper insights on the fundamental regulation of zygotic genome activation( ZGA). Written in the highly successful Methods in Molecular Biology series format, the chapters include brief introductions to the material, lists of necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and a Notes section which highlights tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Zygotic Genome Activation: Methods and Prools aims to serve as a practical reference for researchers interested in early development and genome activation.

Product Details

ISBN-13:	9781071645215
Publisher:	Springer US
Publication date:	05/27/2025
Series:	Methods in Molecular Biology , #2923
Pages:	264
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Probing the genomic distribution of histone modifications in egg extract treated sperm nuclei.- Canonical and Variant Nucleosome Reprogramming from Sperm to Blastula.- Chromatin analyses in mouse oocytes and preimplantation embryos.- Hi-C prool for studying chromatin spatial organization in early zebrafish development.- HiChIP to Study 3D Genome Organization in Xenopus tropicalis Embryos.- CUT&Tag for transcription factor binding profiles during mammalian zygotic genome activation.- Imaging nuclear clusters in live zebrafish embryos.- Quantifying transcription factor enrichment at target loci in live embryos.- Quantifying Nascent Transcription in Early Embryogenesis.- Ribosomal RNA depletion for poly(A)-tail-independent quantification of genome activation.- Monitoring miRNA expression and activity in mammalian oocytes and early embryos.- Ribo-ITP: A method for ribosome profiling in single cells and low input samples.- Imaging Translation in Early Embryo Development.- Image segmentation software for nuclear segmentation and cell cycle tracking from 4D embryo light sheet data.- Quantitative Staging of Mid-blastula Zebrafish Embryos by Nuclei Counting.