Table of Contents

Introduction: B.W. Matthews, Recent Transformations in Structural Biology. Crystals: P.C. Weber, Overview of Protein Crystallization Methods. M. Rigs-Kautt and A. Ducruix, Inferences Drawn from Physicochemical Studies of Crystallogenesis and Precrystalline State. L. Song and J.E. Gouaux, Membrane Protein Crystallization: Application of Sparse Matrix to alpha-Hemolysin Heptamer. C.W. Carter, Jr., Response Surface Methods for Optimizing and Improving Reproducibility of Crystal Growth. A. George, Y. Chiang, B. Guo, A. Arabshahi, Z. Cai, and W.W. Wilson, Second Virial Coefficient as Predictor in Protein Crystal Growth. J.R. Luft and G.T. DeTitta, Kinetic Aspects of Macromolecular Crystallization. R. Sousa, Using Cosolvents to Stabilize Protein Conformation for Crystallization. C.E. Kundrot, Preparation and Crystallization of RNA: A Sparse Matrix Approach. A.R. Ferre-Damare and S.K. Burley, Dynamic Light Scattering in Evaluating Crystallizability of Macromolecules. A.M. Edwards, S.A. Darst, S.A. Hemming, F.J. Asturias, P.R. David, and R.D. Kornberg, Two-Dimensional Protein Crystals in Aid of Three-Dimensional Protein Crystal Growth. I. Rayment, Reductive Alkylation of Lysine Residues to Alter Crystallization Properties of Proteins. Data: Cryocrystallography: D. Rodgers, Practical Cryocrystallography. J.R. Helliwell, Overview of Synchrotron Radiation and Macromolecular Crystallography. Z.R. Korszun, Neutron Macromolecular Crystallography. Detectors:Y. Amemiya, X-Ray Storage-Phosphor Imaging-Plate Detectors: High-Sensitivity X-Ray Area Detector. E.M. Westbrook and I. Naday, Charge-Coupled Device-Based Area Detectors. R. Kahn and R. Fourme, Gas Proportional Detectors. Data Collection Software:J.W. Pflugrath, Diffraction-Data Processing for Electronic Detectors: Theory and Practice. Z. Otwinowski and W. Minor, Processing of X-Ray Diffraction Data Collected in Oscillation Mode. Z. Dauter, Data Collection Strategy. T.O. Yeates, Detecting and Overcoming Crystal Twinning. Phases: Basic Concepts:G. Bricogne, Bayesian Statistical Viewpoint on Structure Determination: Basic Concepts and Examples. G. Bricogne, Efficient Sampling Methods for Combinations of Signs, Phases, Hyperphases, and Molecular Orientations. H. Ke, Overview of Isomorphous Replacement Phasing. M.A. Rould, Screening for Heavy-Atom Derivatives and Obtaining Accurate Isomorphous Differences. E. de La Fortelle and G. Bricogne, Maximum-Likelihood Heavy-Atom Parameter Refinement for Multiple Isomorphous Replacement and Multiwavelength Anomalous Diffraction Methods. Multiwavelength Methods:W.A. Hendrickson and C.M. Ogata, Phase Determination by Multiwavelength Anomalous Diffraction Methods. S. Doublie, Preparation of Selenomethionyl Proteins for Phase Determination. T.C. Terwilliger, Multiwavelength Anomalous Diffraction Phasing of Macromolecular Structures: Analysis of MAD Data as Single Isomorphous Replacement with Anomalous Scattering Data Using MADMRG Program. V. Ramakrishnan and V. Biou, Treatment of Multiwavelength Anomalous Diffraction Data as a Special Case of Multiple Isomorphous Replacement. Molecular Replacement:A.T. Branger, Patterson Correlation Searches and Refinement. J. Navaza and P. Saludjian, AMoRe: An Automated Molecular Replacement Program Package. L. Tong and M. Rossmann, Rotation Function Calculations with GLRF Program. G.A. Bentley, Phased Translation Function. Horizon MethodsJ.M. Berg and N.W. Goffeney Centrosymmetric Crystals of Biomolecules: The Racemate Method. G.M. Sheldrick, Patterson Superposition and ab Initio Phasing. A.D. Podjarny and A.G. Urzhumtsev, Low-Resolution Phasing. Author Index. Subject Index.