Plants from Test Tubes: An Introduction to Micropropogation

Thirty years ago, in vitro propagation was a new technique for producing plants, and Lydiane Kyte’s Plants from Test Tubes became the standard work on the topic.

The new fourth edition has been thoroughly revised and updated to reflect the many advances in science and technology, including the five accepted sequential stages of micropropagation. Ten new plants have been added. This in turn has greatly expanded the already extensive bibliography. Among the new topics that have been introduced or expanded on are embryo culture for breeding, somaclonal variation, anther culture, somatic embryogenesis, cryopreservation, and genetic engineering. More ornamental plant examples are given and many new illustrations provided, including a chronology of discoveries in micropropagation.

1134864028
Plants from Test Tubes: An Introduction to Micropropogation

Thirty years ago, in vitro propagation was a new technique for producing plants, and Lydiane Kyte’s Plants from Test Tubes became the standard work on the topic.

The new fourth edition has been thoroughly revised and updated to reflect the many advances in science and technology, including the five accepted sequential stages of micropropagation. Ten new plants have been added. This in turn has greatly expanded the already extensive bibliography. Among the new topics that have been introduced or expanded on are embryo culture for breeding, somaclonal variation, anther culture, somatic embryogenesis, cryopreservation, and genetic engineering. More ornamental plant examples are given and many new illustrations provided, including a chronology of discoveries in micropropagation.

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Plants from Test Tubes: An Introduction to Micropropogation

Plants from Test Tubes: An Introduction to Micropropogation

Plants from Test Tubes: An Introduction to Micropropogation

Plants from Test Tubes: An Introduction to Micropropogation

eBookFourth Edition, Revised (Fourth Edition, Revised)

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Overview

Thirty years ago, in vitro propagation was a new technique for producing plants, and Lydiane Kyte’s Plants from Test Tubes became the standard work on the topic.

The new fourth edition has been thoroughly revised and updated to reflect the many advances in science and technology, including the five accepted sequential stages of micropropagation. Ten new plants have been added. This in turn has greatly expanded the already extensive bibliography. Among the new topics that have been introduced or expanded on are embryo culture for breeding, somaclonal variation, anther culture, somatic embryogenesis, cryopreservation, and genetic engineering. More ornamental plant examples are given and many new illustrations provided, including a chronology of discoveries in micropropagation.


Product Details

ISBN-13: 9781604695458
Publisher: Timber Press, Incorporated
Publication date: 04/08/2025
Sold by: Barnes & Noble
Format: eBook
Pages: 272
File size: 7 MB

About the Author

Lydiane (Ann) Kyte holds a B.S. degree in botany from the University of Washington. She built a plant tissue culture program for Briggs Nursery, now world famous for its tissue-cultured plants.
John Kleyn received a Ph.D. in bacteriology from Cornell University and currently acquires books for Indonesian Universities.

Holly Scoggins teaches horticulture at Virginia Tech in Blacksburg. Her courses cover ornamental plant production and marketing, herbaceous landscape plants, and greenhouse management. Her research and extension focus includes production of perennials and field trials of hops. She holds a PhD in horticultural science from North Carolina State University.


Dr. Mark Bridgen is a professor at Cornell University and director of the Long Island Horticultural Research and Extension Center. He earned a PhD in plant physiology from Virginia Polytechnic Institute and State University. Dr. Bridgen teaches plant propagation, and his research specializes in ornamental plant breeding, plant tissue culture and micropropagation, and floriculture.

Table of Contents

Preface to the Fourth Edition 8

Preface to the Third Edition 9

Preface to the First and Second (Revised) Editions 10

Section 1 The Basics of Tissue Culture

1 Overview 12

2 The Botanical Basis for Tissue Culture 21

3 History 28

4 The Laboratory: Design, Equipment, and Supplies 38

5 Media Ingredients 60

6 Media Preparation 79

7 Explants: Selecting, Managing, and Preparing Stock 89

8 Sterile Technique and Aseptic Culture 96

9 The Growing Room: Plant Multiplication and Dealing with Problems 107

10 Rooting and Preparing for Transplant 115

11 Dealing with Biological Contaminants 122

12 Business 138

13 Plant Biotechnology 149

Section 2 Culture Guide to Selected Plants

Actinidia deliciosa, kiwifruit 172

Alstroemeria hybrids, Inca lily 173

Amelanchier alnifolia, serviceberry 174

Anihurium andraeanum, flamingo flower 174

Asparagus officinalis, asparagus 175

Begonia rex-cultorum, rex begonia 176

Brassica oleracea var. italica and var. botrytis, broccoli and cauliflower 177

Catharanthus roseus, Madagascar periwinkle 178

Cattleya spp. and hybrids, cattleya 179

Chrysanthemum xgrandiflorum, garden mum 181

Citrus xlimon, lemon 181

Corymhiaficifolia, red-flowering gum 182

Cucumis sativus, garden cucumber 183

Cymbidium spp. and hybrids, cymbidium 183

Dianthus caryophyllus, carnation 184

Dieffenbachia seguine, dumb cane 185

Epiphyllum chrysocardium, heart of gold 186

Ficus carica, fig 187

Fragaria xananassa, garden strawberry 188

Freesia xhybrida, freesia 188

Gerbera jamesonii, Transvaal daisy 189

Hemerocallis spp. and hybrids, daylily 190

Heuchera spp. and hybrids, alumroot 191

Hippeastrum spp., amaryllis, 191

Hosta spp. and hybrids, hosta 192

Hypoxis hemerocallidea, African potato 193

Ipomoea batatas, sweet potato vine 194

Iris germanica (rhizomatous), bearded iris 195

Iris xhollandica (bulbous), Dutch iris 196

Juglans regia, English walnut 196

Kalanchoe blossfeldiana, kalanchoe 197

Kalmia latifolia, mountain laurel 198

Lilium spp., lily 199

Malus domestica, apple 200

Mammillaria elongata, golden star cactus 201

Miltonia, Odontoglossum, and Oncidium, miltonia, odontoglossum, and oncidium 201

Miscanihus sinensis, Chinese silver grass 202

Musa acuminata x M. balbisiana, banana 203

Nandina domestica, heavenly bamboo 204

Nephrolepis exaltata, Boston fern 204

Panax ginseng and P. quinquefolius, Asian ginseng and American ginseng 205

Pelargonium graveolens, scented geranium 206

Phalaenopsis spp. and hybrids, moth orchid 207

Phlox subulata, moss pink 208

Phyllostachys aurea, golden bamboo 208

Pinus spp., pine 209

Platycerium stemaria, staghorn fern 210

Primula vulgaris, primrose 211

Prunus spp., plum, peach, apricot, and cherry 211

Rhododendron spp. and hybrids, rhododendron and azalea 212

Rosa spp. and hybrids, rose 214

Rubus fruticosus, blackberry 214

Rubus occidentalis, American raspberry 215

Saintpaulia ionantha, African violet 216

Sequoia sempervirens, coastal redwood 217

Solanum tuberosum, potato 217

Syngonium podophyllum, arrowhead vine 218

Syringa spp. and hybrids, lilac 219

Vaccinium corymbosum, highbush blueberry 220

Vitis vinifera, wine grape 221

Section 3 Resources

Formula Comparison Table 224

Conversion Formulas and Tables 226

The Microscope: Introduction, Use, and Care 228

Professional Organizations 232

Suppliers 232

Glossary 236

Bibliography 242

Photo and Illustration Credits 259

Index 260

About the Authors 269

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