Basic Laboratory Methods for Biotechnology / Edition 2

by Lisa Seidman

Overview

Presented from the perspective of the biotech industry, this laboratory handbook/textbook reference gives a systematic, understandable, and practical introduction to fundamental laboratory methods and provides a foundation upon which students can build a career in the lab. The authors balance background and theory with practical information, drawing material from many sources: analytical chemistry texts, molecular biology manuals, industry standards, government regulations, manufacturer and supplier information, and the useful laboratory “lore” that is part of the industry’s oral tradition. KEY TOPICS: The Modern Biotechnology Industry: A Broad Overview, The Business of Biotechnology: The Transformation of Knowledge into Products, Pharmaceutical/Biopharmaceutical Products, Introduction to Product Quality Systems, Biotechnology and the Regulation of Food and Medical Products, Documentation, the Foundation of Quality, Quality Systems in the Production Facility, Quality Systems in the Laboratory, Introduction to a Safe Workplace, Working Safely in the Laboratory: General Considerations and Physical Hazards, Working Safely with Chemicals, Working Safely with Biological Materials, Basic Math Techniques, Proportional Relationships, Relationships and Graphing, Descriptions of Data (Descriptive Statistics),Introduction to Quality Laboratory Measurements, Tests and Assays, Introduction to Instrumental Methods and Electricity,The Measurement of Weight, The Measurement of Volume, The Measurement of Temperature, The Measurement of pH, Selected Ions and Conductivity, Measurements Involving Light A. Basic Principles and Instrumentation, Introduction to Quality Laboratory Tests and Assays, Measurements Involving Light B. Applications and Methods, Preparation of Laboratory Solutions A: Concentration Expressions and Calculations, Preparation of Laboratory Solutions B. Basic Procedures and Practical Information, Solutions: Associated Procedures and Information, Laboratory Solutions to Support the Activity of Biological Macromolecules, Culture Media for Intact Cells, Introduction to Filtration, Introduction to Centrifugation, Introduction to Bioseparations, Computers: An Overview, Data Handling with Computers, Applications of the Internet to Biotechnology. MARKET: Itended for those interested in learning the basics of laboratory methods for biotechnology

Product Details

ISBN-13:	9780321570147
Publisher:	Pearson Education
Publication date:	10/29/2008
Series:	Symbiosis: the Pearson Custom Library for the Biological Sciences Series
Edition description:	New Edition
Pages:	800
Product dimensions:	8.40(w) x 10.90(h) x 1.30(d)

About the Author

Lisa Seidman received her Ph.D. from the University of Wisconsin and has taught for more than 20 years in the Biotechnology Laboratory Technician Program at Madison Area Technical College in Madison, Wisconsin. Cynthia Moore received her Ph.D. in Microbiology from Temple University School of Medicine. She is currently an Associate Professor of Biological Sciences at Illinois State University, where she also serves as Director of Biology Teacher Education.

Table of Contents

I. INTRODUCTION TO THE BIOTECHNOLOGY WORK PLACE

1. The Modern Biotechnology Industry: A Broad Overview

2. The Business of Biotechnology: The Transformation of Knowledge into Products

3. Pharmaceutical/Biopharmaceutical Products

II. PRODUCT QUALITY AND BIOTECHNOLOGY

4. Introduction to Product Quality Systems

5. Biotechnology and the Regulation of Food and Medical Products

6. Documentation, the Foundation of Quality

7. Quality Systems in the Production Facility

8. Quality Systems in the Laboratory

III. SAFETY IN THE LABORATORY

9. Introduction to a Safe Workplace

10. Working Safely in the Laboratory: General Considerations and Physical Hazards

11. Working Safely with Chemicals

12. Working Safely with Biological Materials

IV. MATH IN THE BIOTECHNOLOGY LABORATORY: AN OVERVIEW

13. Basic Math Techniques

14. Proportional Relationships

15. Relationships and Graphing

16. Descriptions of Data (Descriptive Statistics)

V. LABORATORY MEASUREMENTS

17. Introduction to Quality Laboratory Measurements, Tests and Assays

18. Introduction to Instrumental Methods and Electricity

19. The Measurement of Weight

20. The Measurement of Volume

21. The Measurement of Temperature

22. The Measurement of pH, Selected Ions and Conductivity

23. Measurements Involving Light A. Basic Principles and Instrumentation

VI. QUALITY ASSAYS AND TESTS

24. Introduction to Quality Laboratory Tests and Assays

25. Measurements Involving Light B. Applications and Methods

VII. LABORATORY SOLUTIONS

26. Preparation of Laboratory Solutions A: Concentration Expressions and Calculations

27. Preparation of Laboratory Solutions B. Basic Procedures and Practical Information

28. Solutions: Associated Procedures and Information

29. Laboratory Solutions to Support the Activity of Biological Macromolecules

30. Culture Media for Intact Cells

VII. BASIC SEPARATION METHODS

30. Introduction to Filtration

31. Introduction to Centrifugation

32. Introduction to Bioseparations

IX. COMPUTERS IN THE LABORATORY

33. Computers: An Overview

34: Data Handling with Computers

35. Applications of the Internet to Biotechnology