Basic Principles of Drug Discovery and Development

by Benjamin E. Blass

Overview

Basic Principles of Drug Discovery and Development presents the multifaceted process of identifying a new drug in the modern era, providing comprehensive explanations of enabling technologies such as high throughput screening, structure based drug design, molecular modeling, pharmaceutical profiling, and translational medicine, all areas that have become critical steps in the successful development of marketable therapeutics. The text introduces the fundamental principles of drug discovery and development, also discussing important drug targets by class, in vitro screening methods, medicinal chemistry strategies in drug design, principles in pharmacokinetics and pharmacodynamics, animal models of disease states, clinical trial basics, and selected business aspects of the drug discovery process. It is designed to enable new scientists to rapidly understand the key fundamentals of drug discovery, including pharmacokinetics, toxicology, and intellectual property." - Provides a clear explanation of how the pharmaceutical industry works - Explains the complete drug discovery process, from obtaining a lead, to testing the bioactivity, to producing the drug, and protecting the intellectual propertyIdeal for anyone interested in learning about the drug discovery process and those contemplating careers in the industry - Explains the transition process from academia or other industries

Product Details

ISBN-13:	9780124115255
Publisher:	Elsevier Science & Technology Books
Publication date:	04/24/2015
Sold by:	Barnes & Noble
Format:	eBook
Pages:	580
File size:	27 MB
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About the Author

As an industrial medicinal chemist, Dr. Blass has experience with major pharmaceutical organizations (Wyeth, Procter & Gamble Pharmaceuticals) and small biotech operations (Fox Chase Chemical Diversity Center), which provided him with a wealth of expertise in the art of drug discovery and development (including a wide range of disease states and biological targets). His position with Temple University's School of Pharmacy and the Moulder Center for Drug Discovery has provided him with essential experience as an educator and academic scientist. These experiences, as well as his training and expertise as a registered US patent agent, have provided him with the tools and skills necessary to bridge the gap between industrial drug discovery and academic research.
As an industrial medicinal chemist, Dr. Blass has experience with major pharmaceutical organizations (Wyeth, Procter & Gamble Pharmaceuticals) and small biotech operations (Fox Chase Chemical Diversity Center), which provided him with a wealth of expertise in the art of drug discovery and development (including a wide range of disease states and biological targets). His position with Temple University’s School of Pharmacy and the Moulder Center for Drug Discovery has provided him with essential experience as an educator and academic scientist. These experiences, as well as his training and expertise as a registered US patent agent, have provided him with the tools and skills necessary to bridge the gap between industrial drug discovery and academic research.

Table of Contents

Foreword1. Drug Discovery and Development: An Overview of Modern Methods and PrinciplesDrug Discovery and Development from 20,000 FeetTarget Selection: The First Step ForwardHit Identification: Finding a Starting PointIdentify a Clinical Candidate: Juggling the PropertiesQuestionsReferences2. The Drug Discovery Process: From Ancient Times to the Present DayThe Age of Botanicals: Preindustrial Drug DiscoveryPaul Ehrlich: The Father of Modern Drug DiscoveryMilestones in Drug Discovery Milestones in Animal Models: Breeding a Better Model Milestones in Molecular Science X-ray Crystallography Molecular Modeling and Computational Chemistry High Throughput Technology: Chemical Synthesis and Screening Science Milestones in Biotechnology Recombinant DNA and Transfection Technology Polymerase Chain Reaction (PCR) Technology Monoclonal Antibody and Hybridoma TechnologyThe Rise of Biologics and Macromolecular TherapeuticsSocietal and Governmental Impacts The Pure Food and Drug Act of 1906 The Elixir of Sulfanilamide Disaster of 1937 The Thalidomide StoryRegulatory Milestones Durham–Humphrey Amendment of 1951 Kefauver–Harris Amendment of 1962 Hatch–Waxman Act of 1984 Biologics Price Competition and Innovation Act of 2009Future Developments in Drug DiscoveryQuestionsReferences3. Classical Targets in Drug DiscoveryProtein StructureEnzymesInhibition of EnzymesG-Protein-Coupled Receptors (GPCRs) G-Protein-Dependent Signaling pathways cAMP Signaling IP3 Signaling Modulating GPCR ActivityIon Channels Gating Mechanisms Ligand-Gated Channels Voltage-Gated Channels Other Gating MechanismsMembrane Transport Proteins (Transporters)Emerging TargetsQuestionsReferences4. In Vitro Screening SystemsThe Language of Screening: Basic Terms Concentration Response Curves and IC50s Dissociation Constants (Kd) and Inhibition Constants (Ki) Efficacy versus Binding: EC50s Agonist, Partial Agonist, Antagonist, Allosteric Modulators, and Inverse Agonists Agonists and Partial Agonists Antagonists Basal Activity and Inverse Agonists Allosteric Modulation Receptor ReserveStreptavidin and BiotinBiochemical versus Cellular AssaysAssay Systems and Methods of DetectionRadioligand Systems Scintillation Proximity Assay (SPA)Enzyme-Linked Immunosorbent Assay (ELISA)Fluorescence-Based Assay Systems Fluorescence Polarization (FP) Fluorescence Resonance Energy Transfer (FRET) Time-Resolved Fluorescence Resonance Energy Transfer (TRFRET) Amplified Luminescent Proximity Homogeneous Assay (AlphaScreen™) Fluorescent Detection of Calcium FluxReporter Gene Assays Chloramphenicol Acetyltransferase (CAT) β-Lactamase Reporter Assays Luciferase Reporter AssaysKinetic Fluorescent Measurement SystemsLabel-Free Assay Systems Cellular Dielectric Spectroscopy Optical Biosensors Surface Plasmon Resonance TechnologyElectrophysiological Patch ClampGeneral Consideration for All Screening MethodsQuestionsReferences5. Medicinal ChemistryStructure–Activity Relationships and Structure–Property RelationshipsThe Role of ChiralityPush and Pull in Structure–Activity RelationshipsQuantitative Structure–Activity RelationshipsThe PharmacophoreDeveloping an SAR Data SetThe Structure–Activity Relationship CycleBioisosterismStructure–Activity Relationship, Selectivity and Physicochemical Properties"Druglike GuidelinesQuestionsReferences6. In vitro ADME and In vivo PharmacokineticsAbsorption Solubility PermeabilityDistribution Permeability Transporters Plasma Protein BindingElimination Pathways Metabolism ExcretionIn vitro ADME Screening MethodsIn Vivo Pharmacokinetics Volume of Distribution Clearance Half-life BioavailabilitySpecies selectionQuestionsReferences7.

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From the Publisher

Presents the multidisciplinary process of identifying a new drug, including perspectives from medicinal chemists, biologists, pharmacologists, drug metabolism experts, toxicologists, and clinicians