Cytokine Protocols

Cytokine Protocols

by Marc De Ley (Editor)

Hardcover(2nd ed. 2012)

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Product Details

ISBN-13: 9781617794384
Publisher: Springer-Verlag New York, LLC
Publication date: 12/01/2011
Series: Methods in Molecular Biology , #820
Edition description: 2nd ed. 2012
Pages: 241
Product dimensions: 7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

1 A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods can Engender Discovery

Alfons Billiau

2The use of Real-Time Quantitative PCR (RT-qPCR) for the Analysis of Cytokine mRNA Levels

Maria Forlenza, Thomas Kaiser, Huub F.J. Savelkoul, and Geert F. Wiegertjes

3Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles using Affymetrix Microarray and DAVID

Tomozumi Imamichi, Jun Yang, Da Wei Huang, Brad Sherman, and Richard A. Lempicki

4Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues

Markus Bitzer, Wenjun Ju, Xiahong Jing and Jiri Zavadil

5 Evaluating Posttranscriptional Regulation of Cytokine Genes

Bernd Rattenbacher and Paul R. Bohjanen

6Cloning of Cytokine 3' Untranslated Regions and Post-Transcriptional Assessment using Cell-Based GFP Assay

Latifa Al-Haj and Khalid S.A. Khabar

7Integrin Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs in vitro and in vivo

Charudharshini Srinivasan, Dan Peer, and Motomu Shimaoka

8 Hammerhead Ribozyme Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth Factor

Paulette M. Robinson, Timothy D. Blalock, Rong Yuan, Alfred S. Lewin, and Gregory S. Schultz

9 Control of the Interferon Response in RNAi Experiments

Jana Nejepinska, Matyas Flemr, and Petr Svoboda

10. shRNA-induced Interferon-Stimulated Gene Analysis

Núria Morral and Scott R. Witting

11Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells

Fabrice Moore, Daniel. A. Cunha, Hindrik Mulder, and Decio L. Eizirik

12Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins

Daniela Novick and Menachem Rubinstein

13 In Vitro Stimulation and Detection of IFNa Production in Human Plasmacytoid Dendritic Cells

William C. Adams and Karin Loré

14 A Sensitive and Versatile Cytokine Bioassay Based on Type I interferon Signaling in 2fTGH Cells

Lennart Zabeau, José Van der Heyden, and Jan Tavernier

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