Cytokine Protocols

by Marc De Ley (Editor)

Overview

In these 19 chapters, researchers, physicians, and clinicians describe each step of their techniques for examining and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Each protocol includes a background introduction, equipment and reagent lists, and, where needed, advice on the interpretation of results. Annotation ©2004 Book News, Inc., Portland, OR

Product Details

ISBN-13:	9781617794384
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/01/2011
Series:	Methods in Molecular Biology , #820
Edition description:	2nd ed. 2012
Pages:	241
Product dimensions:	7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

1 A Tale of Two Interferon Bioassays: How Frustration with Discrepant Results from Slightly Dissimilar Methods can Engender Discovery

Alfons Billiau

2The use of Real-Time Quantitative PCR (RT-qPCR) for the Analysis of Cytokine mRNA Levels

Maria Forlenza, Thomas Kaiser, Huub F.J. Savelkoul, and Geert F. Wiegertjes

3Interleukin-27 Induces Interferon-Inducible Genes: Analysis of Gene Expression Profiles using Affymetrix Microarray and DAVID

Tomozumi Imamichi, Jun Yang, Da Wei Huang, Brad Sherman, and Richard A. Lempicki

4Quantitative Analysis of miRNA Expression in Epithelial Cells and Tissues

Markus Bitzer, Wenjun Ju, Xiahong Jing and Jiri Zavadil

5 Evaluating Posttranscriptional Regulation of Cytokine Genes

Bernd Rattenbacher and Paul R. Bohjanen

6Cloning of Cytokine 3' Untranslated Regions and Post-Transcriptional Assessment using Cell-Based GFP Assay

Latifa Al-Haj and Khalid S.A. Khabar

7Integrin Targeted Stabilized Nanoparticles for an Efficient Delivery of siRNAs in vitro and in vivo

Charudharshini Srinivasan, Dan Peer, and Motomu Shimaoka

8 Hammerhead Ribozyme Mediated Knockdown of mRNA for Fibrotic Growth Factors: Transforming Growth Factor-Beta 1 and Connective Tissue Growth Factor

Paulette M. Robinson, Timothy D. Blalock, Rong Yuan, Alfred S. Lewin, and Gregory S. Schultz

^{9 Control of the Interferon Response in RNAi Experiments}

^{Jana Nejepinska, Matyas Flemr, and Petr Svoboda}

^10. shRNA-induced Interferon-Stimulated Gene Analysis

Núria Morral and Scott R. Witting

11Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells

Fabrice Moore, Daniel. A. Cunha, Hindrik Mulder, and Decio L. Eizirik

^{12Ligand Affinity Chromatography, an Indispensable Method for the Purification of Soluble Cytokine Receptors and Binding Proteins}

^{Daniela Novick and Menachem Rubinstein}

13 In Vitro Stimulation and Detection of IFNa Production in Human Plasmacytoid Dendritic Cells

William C. Adams and Karin Loré

14 A Sensitive and Versatile Cytokine Bioassay Based on Type I interferon Signaling in 2fTGH Cells

Lennart Zabeau, José Van der Heyden, and Jan Tavernier