Cytokine Protocols

by Marc De Ley (Editor)

Overview

"Recent studies have discovered new known and characterized cytokines, allowing for advancement in miniaturization of micro-analytical methods as well as the extensive development of bio-informatics and nanotechnology. These advancements have allowed researchers to reduces sample sizes making for more accurate determinations then previously possible. In Cytokine Protocols: Second Edition, expert researchers in the field detail many of the methods which are now commonly used to study cytokines. These methods and techniques for studying cytokines include historical importance and the importance of researchers using bioassay, quantification, and characterization of cytokine related RNAs, posttranscriptional modifications of RNA, either naturally or artificially, and observations at the protein level. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Authoritative and practical, Cytokine Protocols: Second Edition seeks to aid scientists in furthering the crucially important advancement of cytokine research."

Product Details

ISBN-13:	9781493958108
Publisher:	Springer-Verlag New York, LLC
Publication date:	08/23/2016
Series:	Methods in Molecular Biology , #820
Edition description:	Softcover reprint of the original 2nd ed. 2012
Pages:	241
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Large-Scale Generation of Plasmids that Express Type I Interferon.- Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay.- Modulation of the Interferon-β Signal by Transfection of Cells with an Antisense-RNA Expressing Vector.- Competitive RT-PCR to Quantify Small Amounts of mRNA.- In SituHybridization for Cytokines in Human Tissue Biopsies.- Purification, Identification, and Synthesis of Chemokines.- Receptor Isolation and Characterization.- Crystallization of Cytokine-Receptor Complexes.- Biosensor Analysis of Receptor-Ligand Interactions.- Analysis of SH2 Ligands and Identification of Sites of Interaction.- Assays for Antiviral Activity.- Assays for Cytotoxicity.- Assays for Chemotaxis.- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor.- Development of a Mammalian Tet-On Expression Cell Line.- Immunohishemical Detection of Cytokines in Human Tissue Sections.- Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level.- Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation.- Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.