Cytokine Protocols

by Marc De Ley (Editor)

Overview

In these 19 chapters, researchers, physicians, and clinicians describe each step of their techniques for examining and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Each protocol includes a background introduction, equipment and reagent lists, and, where needed, advice on the interpretation of results. Annotation ©2004 Book News, Inc., Portland, OR

Product Details

ISBN-13:	9781617794384
Publisher:	Springer-Verlag New York, LLC
Publication date:	12/01/2011
Series:	Methods in Molecular Biology , #820
Edition description:	2nd ed. 2012
Pages:	241
Product dimensions:	7.01(w) x 10.00(h) x 0.03(d)

Table of Contents

Large-Scale Generation of Plasmids that Express Type I Interferon.- Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay.- Modulation of the Interferon-β Signal by Transfection of Cells with an Antisense-RNA Expressing Vector.- Competitive RT-PCR to Quantify Small Amounts of mRNA.- In SituHybridization for Cytokines in Human Tissue Biopsies.- Purification, Identification, and Synthesis of Chemokines.- Receptor Isolation and Characterization.- Crystallization of Cytokine-Receptor Complexes.- Biosensor Analysis of Receptor-Ligand Interactions.- Analysis of SH2 Ligands and Identification of Sites of Interaction.- Assays for Antiviral Activity.- Assays for Cytotoxicity.- Assays for Chemotaxis.- In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor.- Development of a Mammalian Tet-On Expression Cell Line.- Immunohishemical Detection of Cytokines in Human Tissue Sections.- Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level.- Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation.- Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production.