Table of Contents
Preface v
Contributors ix
1 Flow Cytometry: An Introduction Alice L. Givan 1
2 Breaking the Dimensionality Barrier C. Bruce Bagwell 31
3 Quantitative Fluorescence Measurements with Multicolor Flow Cytometry Lili Wang Adolfas K. Gaigalas Ming Yan 53
4 Quantum Dots for Quantitative Flow Cytometry Tione Buranda Yang Wu Larry A. Sklar 67
5 Bead-Based Multiplexed Analysis of Analytes by Flow Cytometry Henri C. van der Heyde Irene Gramaglia 85
6 Flow-Based Combinatorial Antibody Profiling: An Integrated Approach to Cell Characterization Shane Bruckner Ling Wang Ruiling Yuan Perry Haaland Amitabh Gaur 97
7 Tracking Immune Cell Proliferation and Cytotoxic Potential Using Flow Cytometry Joseph D. Tario Jr. Katharine A. Muirhead Dalin Pan Mark E. Munson Paul K. Wallace 119
8 Multiparameter Intracellular Cytokine Staining Patricia Lovelace Holden T. Maecker 165
9 Phospho Flow Cytometry Methods for the Analysis of Kinase Signaling in Cell Lines and Primary Human Blood Samples Peter O. Krutzik Angelica Trejo Kenneth R. Schulz Garry P. Nolan 179
10 Multiparametric Analysis of Apoptosis by Flow Cytometry William G. Telford Akira Komoriya Beverly Z. Packard C. Bruce Bagwell 203
11 Multiparameter Cell Cycle Analysis James W. Jacobberger R. Michael Sramkoski Tammy Stefan 229
12 Rare Event Detection and Analysis in Flow Cytometry: Bone Marrow Mesenchymal Stem Cells, Breast Cancer Stem/Progenitor Cells in Malignant Effusions, and Pericytes in Disaggregated Adipose Tissue Ludovic Zimmerlin Vera S. Donnenberg Albert D. Donnenberg 251
13 Flow Cytometry-Based Identification of Immature Myeloerythroid Development Cornelis J.H. Pronk David Bryder 275
14 Flow Cytometry Immunophenotyping of Hematolymphoid Neoplasia Katherine R. Calvo Catharine S. McCoy Maryalice Stetler-Stevenson 295
15 Flow Cytometry Assays in Primary Immunodeficiency Diseases Maurice R. G. O'Gorman Joshua Zollett Nicolas Bensen 317
16 Flow Cytometric Analysis of Microparticles Henri C. van der Heyde Irene Gramaglia Valéry Combes Thaddeus C. George Georges E. Grau 337
17 Noncytotoxic DsRed Derivatives for Whole-Cell Labeling Rita L. Strack Robert J. Keenan Benjamin S. Glick 355
18 Flow Cytometric FRET Analysis of Protein Interaction György Vereb Péter Nagy János Szöllosi 371
19 Fluorescent Protein-Assisted Purification for Gene Expression Profiling M. Raza Zaidi Chi-Ping Day Glenn Merlino 393
20 Multiparametric Analysis, Sorting, and Transcriptional Profiling of Plant Protoplasts and Nuclei According to Cell Type David W. Galbraith Jaroslav Janda Georgina M. Lambert 407
21 Lentiviral Fluorescent Protein Expression Vectors for Biotinylation Proteomics Irene Riz Teresa S. Hawley Robert G. Hawley 431
22 Standard Practice for Cell Sorting in a BSL-3 Facility Stephen P. Perfetto David R. Ambrozak Richard Nguyen Mario Roederer Richard A. Koup Kevin L. Holmes 449
23 The Cytometric Future: It Ain't Necessarily Flow! Howard M. Shapiro 471
Index 483
