Gel Electrophoresis of Proteins: A Practical Approach / Edition 3

by B. D. Hames

Overview

This new edition is almost a completely new text, with eight of the ten chapters written by new authors. It presents the most reliable methods for essential procedures such as one-dimensional polyacrylamide gel electrophoresis, isoelectric focusing, two-dimensional gel electrophoresis, preparative gel electrophoresis, and peptide mapping, complete with the latest refinements of the procedures. In addition, it describes major new techniques developed since the previous edition. These include capillary gel electrophoresis, sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analysis of protein-protein interactions by gel electrophoresis. Like the previous editions, this volume is easy-to-follow and thorough, a laboratory manual written by experienced researchers for researchers. The emphasis is on describing the best methods, in step-by-step detail, with copious advice to ensure that each method works the first time. This new edition is a 'must' for anyone currently uses gel electrophoresis.

Product Details

ISBN-13:	9780199636402
Publisher:	Oxford University Press
Publication date:	12/10/1998
Series:	Practical Approach Series , #197
Edition description:	REV
Pages:	372
Product dimensions:	6.20(w) x 9.10(h) x 0.80(d)

About the Author

University of Leeds

University of Essex

Table of Contents

1. One-dimensional PAGE2. Protein detection3. Preparative gel electrophoresis4. Capillary SDS electrophoresis5. Conventional isoelectric focusing6. 2-D gel electrophoresis7. Peptide mapping8. Sequence analysis of gel-resolved proteins9. Analysis of glycoproteins10. Protein: protein interactionsAppendix 1 - List of suppliersAppendix 2 - Bibliography of polypeptide detection methods

Editorial Reviews

"A laboratory manual presenting methods in a step-by-step format, and hints and tips from experienced researchers. Among the varieties of gel electrophoresis described are one-dimensional polyacrylamide; isoelectric, two-dimensional; and preparative. Eight of the ten chapters of the earlier editions, in 1981 and 1990, have been rewritten by new authors. They discuss methods that have been developed and tested since then, including capillary gel electrophoresis, the sequence analysis of gel-resolved proteins, fluorophore-labelled saccharide electrophoresis, and analyzing interactions between proteins."—SciTech Book News

From the Publisher