Gene Therapy of Cancer: Methods and Protocols

by Wolfgang Walther (Editor)

Overview

This third edition provides new and updated chapters on gene therapeutic strategies of cancer. Chapters guide readers through suicide and oncolytic gene therapy, gene replacement and gene suppression therapy, vector development and refinement, immunogene therapy, TCR and CAR engineering, tumor vaccination using DNA or RNA vaccines, and antitumoral immune stimulation at different levels. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Gene Therapy of Cancer: Methods and Protocols, Third Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Product Details

ISBN-13:	9781071624418
Publisher:	Springer US
Publication date:	06/22/2022
Series:	Methods in Molecular Biology , #2521
Sold by:	Barnes & Noble
Format:	eBook
File size:	44 MB
Note:	This product may take a few minutes to download.

About the Author

Walther, Wolfgang (Max-Delbruck Center for Molecular Medicine); Stein, Ulrike (Max-Delbruck Center for Molecular Medicine)

Table of Contents

Brief Introduction to Current Cancer Gene Therapy.- Minicircles for CAR T Cell Production by Sleeping Beauty Transposition - A Technological Overview.- Generation of CAR-T Cells with Sleeping Beauty Transposon Gene Transfer.- Generation of Redirected Engineered Human Chimeric Antigen Receptor (CAR) T cells.- TCR- and CAR-engineering of Primary Human T cells.- TCR Gene Therapy for Cancer.- Gene Gun Her2/neu DNA Vaccination: Evaluation of Vaccine Efficacy in a Syngeneic Her2/neu Mouse Tumor Model.- Construction of Minicircle Suicide Genes Coding for Ribosome-Inactivating Proteins.- Claudin-targeted Suicide Gene Therapy for Claudin-Overexpressing Tumor Cells by Using Modified Clostridium Perfringens Enterotoxin (CPE).- Bioengineered RNA Therapy in Patient-Derived Organoids and Xenograft Mouse Models.- Application of Transcription Factor Decoy Oligodeoxynucleotides (ODNs) for Cancer Therapy.- NK Cell Effector Functions and Bystander Tumor Cell Killing in Immunovirotherapy.- A qPCR-based Method for Quantification of Replication Competent Adenovirus (RCA) in Conditionally Replicating Oncolytic Adenoviruses.- Visualized Cancer Immunotherapy with Engineered Salmonella typhimurium.- CANCER Gene Therapy: Development and Production Of Lentiviral Vectors For Gene Therapy.- Capillary Gel Electrophoresis (CGE) for Quality Control of Plasmid DNA in Gene Therapy: quality control of 20-years stored GMP-grade Plasmid DNA.- Efficient Generation of Superior Dumbbell-Shaped Non-viral DNA Delivery Vectors Using 1-2-3 Gap-primer PCR.

Editorial Reviews

3 Stars from Doody

The editors describe in step-by-step fashion different genes and delivery methods to be used for gene therapy of cancer. The purpose is to provide a broad range of protocols for the delivery of therapeutic genes into patients with tumors. This is worthy in a rapidly advancing field and the editors' objectives are met. This book is written for a wide ranging audience including experts in the field of gene therapy as well as medical practitioners. The editors and contributors are credible investigators in their field who have combined not only the basic science methods of transduction, but also introductions explaining the clinical ramifications. A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used. This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients. This book is well written and a translational resource for those interested in the rapidly advancing and novel field of gene therapy for malignancies.

Richard K. Burt

Thirty-nine contributions survey a wide array of the methodologies being currently used and developed for gene therapy for cancer. Representative methods are delineated in immunotherapy and tumor vaccination, suicide gene therapy, antioncogene therapy, and antisense and ribozyme gene therapy. The first 26 papers review experimental approaches utilizing both viral and non-viral gene delivery systems, while Part II links basic research to clinical protocols. Practical guidance is provided for researchers as well as graduate students in this rapidly evolving field. The editors are affiliated with the Max-Delbr<:u>ck Center for Molecular Medicine in Berlin, Germany. Contributors are from the US, Europe, and Japan. Spiral-comb binding. Annotation c. Book News, Inc., Portland, OR (booknews.com)

Booknews

From the reviews of the second edition:

“This book, authored by a number of prominent researchers in the field … provides an overview of recent ground-breaking experimental and clinical concepts and prools in cancer gene therapy. … the editors and authors are to be commended for producing a well-organised, authoritative and succinct coverage of the vast area of gene therapy prools. The materials are relevant, easy-to-follow and well-presented with good illustrations. Without doubt it will serve as a useful resource for those interested in this field, both scientists and clinicians … .”­­­ (Han Hsi Wong and Yaohe Wang, Human Genetics, Vol. 128, July, 2010)

From the Publisher

Reviewer: Richard K. Burt, MD (Northwestern University Feinberg School of Medicine)
Description: The editors describe in step-by-step fashion different genes and delivery methods to be used for gene therapy of cancer.
Purpose: The purpose is to provide a broad range of protocols for the delivery of therapeutic genes into patients with tumors. This is worthy in a rapidly advancing field and the editors' objectives are met.
Audience: This book is written for a wide ranging audience including experts in the field of gene therapy as well as medical practitioners. The editors and contributors are credible investigators in their field who have combined not only the basic science methods of transduction, but also introductions explaining the clinical ramifications.
Features: A wide variety of methods used to deliver genes to tumors are discussed, as are the type of genes used. This includes tumor vaccination, suicide genes, antisense genes, ribozymes, etc. The unique aspect of this book is the translational approach of explaining laboratory techniques as well as the application to patients.
Assessment: This book is well written and a translational resource for those interested in the rapidly advancing and novel field of gene therapy for malignancies.

Doody's Review Service