Laboratory Methods in Enzymology: Protein Part B

by Jon Lorsch (Editor)

Overview

Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. - Indispensable tool for the researcher - Carefully written and edited by experts to contain step-by-step protocols - In this volume we have brought together a number of core protocols concentrating on protein

Product Details

ISBN-13:	9780124201798
Publisher:	Elsevier Science & Technology Books
Publication date:	02/24/2014
Series:	Methods in Enzymology , #539
Sold by:	Barnes & Noble
Format:	eBook
Pages:	240
File size:	9 MB

Table of Contents

Small-scale expression of proteins in E. coliProtein expression-YeastRecombinant protein expression in baculovirus-infected insect cellsSingle cell cloning of a stable mammalian cell lineIn vitro synthesis of proteins in bacterial extractsPreparation of a Saccharomyces cerevisiae cell-free extract for in vitro translationYeast two-hybrid screenUV cross-linking of interacting RNA and protein in cultured cellsAnalysis of RNA–protein interactions by cell mixingGeneral protein–protein cross-linkingChromatin immunoprecipitation and multiplex sequencing (ChIP-Seq) to identify global transcription factor binding sites in the nematode Caenorhabditis elegansPAR-CLIP (photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation): A step-by-step protocol to the transcriptome-wide identification of binding sites of RNA-binding proteinsDetermining the RNA specificity and targets of RNA-binding proteins using a three-hybrid systemDissecting a known RNA–protein interaction using a yeast three-hybrid systemIdentifying proteins that bind a known RNA sequence using the yeast three-hybrid system

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From the Publisher

In this volume we have brought together a number of core protocols concentrating on Protein, carefully written and edited by experts.