Leukemia and Lymphoma: Detection of Minimal Residual Disease / Edition 1

by Theodore F. Zipf, Dennis A. Johnston

Overview

In Leukemia and Lymphoma: Detection of Minimal Residual Disease, hands-on experts describe and discuss the minimal residual disease (MRD) methods they have successfully pioneered for leukemias and lymphomas. They apply reverse transcription PCR (RT-PCR) to acute myeloid leukemia (AML), chronic myelogenous leukemia (CML), and acute promyelocytic leukemia (APL). Other PCR methods are used for Non-Hodgkin's Lymphoma and for the monitoring of follicular lymphoma. Additional chapters address the use of real-time quantitative PCR (RQ-PCR), the emergent method of choice, in patients with acute lymphoblastic leukemia (ALL), the evaluation of MRD techniques in clinical trials, and the application of flow cytometry techniques.

Product Details

ISBN-13:	9781617372797
Publisher:	Springer-Verlag New York, LLC
Publication date:	11/05/2010
Edition description:	Softcover reprint of hardcover 1st ed. 2003
Pages:	257
Product dimensions:	5.90(w) x 8.90(h) x 0.70(d)

Table of Contents

1 Flow-Cytometry Methods for the Detection of Residual Leukemia.- 2 Flow-Cytometry—Based Studies of Minimal Residual Disease in Children with Acute Lymphoblastic Leukemia.- 3 PCR Methods for the Detection of Minimal Residual Disease in Childhood Acute Lymphoblastic Leukemia.- 4 Statistical Considerations in the Analysis of Minimal Residual Disease.- 5 The Application of Minimal Residual Disease Detection Methodology to the Clinical Decision-Making Process.- 6 Measurement of Minimal Residual Disease in Children Undergoing Allogeneic Stem Cell Transplant for Acute Lymphoblastic Leukemia.- 7 Molecular Investigation of Minimal Residual Disease in Adult Acute Lymphoblastic Leukemia of B Lineage.- 8 Investigation of Minimal Residual Disease in Acute Myeloid Leukemia by Immunophenotyping.- 9 Minimal Residual Disease in Acute Myeloid Leukemia: RT-PCR—Based Studies of Fusion Transcripts.- 10 Minimal Residual Disease in Acute Promyelocytic Leukemia.- 11 Minimal Residual Disease in Chronic Myelogenous Leukemia: Results of RT-PCR Detection of BCR-ABL Transcripts.- 12 Utility of PCR Assessment of Minimal Residual Disease After Stem Cell Transplantation in Non-Hodgkin’s Lymphoma.- 13 Real-Time Polymerase Chain Reaction of Genomic DNA for Quantitation of t(14;18).- 14 Monitoring Follicular Lymphoma by Polymerase Chain Reaction.- 15 Editors’ Notes.

Editorial Reviews

"The book provides an excellent presentation of a wide spectrum of methods used in detection of MRD in ALL, CML, AML and Hodgkins lymphoma. It will certainly be of great help for all those, who are engaged in modern management of leukemias and lymphomas." -Neoplasma

From the Publisher