Mononuclear Non-heme Iron Dependent Enzymes Part B

by Jennifer Bridwell-Rabb (Editor)

Overview

Mononuclear Non-heme Iron Dependent Enzymes, Volume 704, Part B focuses on methods for studying, characterizing, and leveraging the chemistry of mononuclear non-heme iron dependent enzymes. Chapters in this new release include Observing extradiol dioxygenases in action through a crystalline lens, Methods used to determine the structure of the oxygenase component of naphthalene 1,2-dioxygenase, Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: biphenyl, phthalate, terephthalate dioxygenase, Substitution of the Mononuclear, Non-heme Iron Cofactor in Lipoxygenases for Structural Studies: Non-heme iron enzyme dependent reactions, and much more.Other sections cover Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxogluatarate dependent enzymes, Expression, purification, and characterization of non-heme Iron-dependent mono-oxygenase OzmD in Oxazinomycin Biosynthesis, Isonitrile Biosynthesis by a Non-Heme Iron(II)-Dependent Oxidases/Decarboxylase, Functional analysis of an a-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesis, Spectroscopic and Computational Studies of a Bifunctional Iron- and 2-oxoglutarate Dependent Enzyme, AsqJ, and much more. - Provides detailed articles regarding how to study the structures and mechanisms of mononuclear non-heme iron dependent enzymes - Guides readers on how to use partner proteins in non-heme iron enzyme catalysis - Includes strategies to employ mononuclear non-heme iron enzymes in biocatalytic applications

Product Details

ISBN-13:	9780443346484
Publisher:	Elsevier Science & Technology Books
Publication date:	09/17/2024
Series:	Methods in Enzymology , #704
Sold by:	Barnes & Noble
Format:	eBook
Pages:	258
File size:	8 MB

Table of Contents

1. Structural studies of mononuclear non-heme iron dependent enzymesYifan Wang2. Observing extradiol dioxygenases in action through a crystalline lensRamaswarmy Subramanian3. Methods used to determine the structure of the oxygenase component of naphthalene 1,2-dioxygenasePravindra Kumar4. Expression, purification, kinetics, and crystallization of non-heme mononuclear iron enzymes: biphenyl, phthalate, and terephthalate dioxygenaseAdam Offenbacher5. Substitution of the Mononuclear, Non-heme Iron Cofactor in Lipoxygenases for Structural Studies: Non-heme iron enzyme dependent reactionsWei-Chen Chang6. Cyclopropanation and aziridination catalyzed by non-heme iron and 2-oxogluatarate dependent enzymesHung-wen (Ben) Liu7. Expression, purification, and characterization of non-heme Iron-dependent mono-oxygenase OzmD in Oxazinomycin BiosynthesisWenjun Zhang8. Isonitrile Biosynthesis by a Non-Heme Iron(II)-Dependent Oxidases/DecarboxylaseIkuro Abe9. Functional analysis of an a-ketoglutarate-dependent non-heme iron oxygenase in fungal meroterpenoid biosynthesisYisong Guo10. Spectroscopic and Computational Studies of a Bifunctional Iron- and 2-oxoglutarate Dependent Enzyme, AsqJShaun McKinnie11. Expression, purification, and biochemical characterization of micro- and macroalgal kaoinoid synthasesJohn Lipscomb12. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylaseTimothy Bugg13. In vitro assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea maysChristopher Schofield14. Methods for production and assaying catalysis of isolated recombinant human aspartate/asparagine--hdyroxylasePaul Fitzpatrick15. Biochemical and Biophysical Approaches to Characterization of the Aromatic Amino Acid Hydroxylases

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From the Publisher

Includes methods to study the remarkable transformations that are facilitated by mononuclear non-heme iron dependent metalloproteins