New Nucleic Acid Techniques / Edition 1

New Nucleic Acid Techniques / Edition 1

by John M. Walker
ISBN-10:
0896031276
ISBN-13:
9780896031272
Pub. Date:
08/31/1988
Publisher:
Springer-Verlag New York, LLC
ISBN-10:
0896031276
ISBN-13:
9780896031272
Pub. Date:
08/31/1988
Publisher:
Springer-Verlag New York, LLC
New Nucleic Acid Techniques / Edition 1

New Nucleic Acid Techniques / Edition 1

by John M. Walker

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Product Details

ISBN-13: 9780896031272
Publisher: Springer-Verlag New York, LLC
Publication date: 08/31/1988
Series: Methods in Molecular Biology , #4
Edition description: 1988
Pages: 560
Product dimensions: 6.10(w) x 9.25(h) x 0.05(d)

Table of Contents

Electrophoresis of RNA Denatured with Glyoxal or Formaldehyde.- Northern Blotting.- Hybrid-Release Translation.- Hybrid-Arrested Translation.- In Vitro Translation and Analysis of Early Events in Protein Synthesis Initiation in Nonreticulocyte Mammalian Cells.- Use of Elutips to Purify DNA.- Plasmid Preparation on Sephacryl S1000.- Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels.- Use of 35S Nucleotides for DNA Sequencing.- Direct Dideoxy DNA Sequencing.- Computer Applications to Molecular Biology.- Detection of Sequence-Specific Protein-DNA Interactions by the DNA-Footprinting Technique.- Oligonucleotide Synthesis Using the Manual Phosphotriester Method.- Manual Oligonucleotide Synthesis Using the Phosphoramidite Method.- Purification of Synthetic Oligonucleotides by Preparative Gel Electrophoresis.- Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries.- Construction of Mammalian Genomic Libraries Using— Replacement Vectors.- Cosmid Library Construction.- Construction of cDNA Libraries in—gt10 or—gt11.- Immunoscreening of—gt11 Expression Libraries Using an Avidin–Biotin Detection System.- Primed Synthesis and Direct Sequencing in the Isolation of cDNA Clones Using Short Oligonucleotides.- Constructing Expression cDNA Libraries Using Unphosphorylated Adaptors.- Expression Screening of cDNA Libraries in pEX.- Producing Antibodies of Predetermined Specificity from Escherichia coli Hybrid Proteins.- Epitope Mapping Using pEX.- DNA Transfection of Mammalian Cells Using Polybrene.- Expression of Foreign Genes in Mammalian Cells.- Dot-Blot Hybridization Method.- Detection of Unique or Low Copy Number DNA Sequences in Complex Genomes.- Photobiotin-Labeled DNA and RNA Hybridization Probes.- Enzyme-Labeled DNA Probes.-Luminescent Detection of Specific DNA Sequences.- Detection of DNA Sequences Using Biotinylated Probes.- Crosslinking of Single-Stranded DNA to Resins.- Extraction of RNA from Plants.- Isolation of Plant Nuclei.- In Vitro Transcription in Plant Nuclei.- Large-Scale Isolation of Ti Plasmid DNA.- Transformation and Regeneration of Dicotyledonous Plants.- Plant Protoplast Fusion.- Plant Tissue Culture.- Direct Gene Transfer into Plant Protoplasts.- Isolation of Wheat Chloroplasts and Chloroplast DNA.- DNA Labeling by Isolated Wheat Chloroplasts.
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