PCR: Methods and Protocols

by Lucília Domingues (Editor)

Overview

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR prools, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory prools, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, PCR: Methods and Prools, Second Edition  aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Product Details

ISBN-13:	9781071633571
Publisher:	Springer US
Publication date:	08/23/2023
Series:	Methods in Molecular Biology , #2967
Edition description:	Second Edition 2023
Pages:	254
Product dimensions:	7.01(w) x 10.00(h) x (d)

Table of Contents

Digital PCR: A partitioning-based application for detection and surveillance of SARS-CoV-2 from sewage samples.- Digital PCR: A tool to authenticate herbal products and spice.- Emulsion Polymerase Chain Reaction coupled with Denaturant Gradient Gel Electrophoresis for microbial diversity studies.- Real-Time PCR High-Resolution Melting Assays for the Detection of Foodborne Pathogens.- High-Throughput real-time qPCR and High-Resolution Melting (HRM) assay for fungal detection in plant matrices.- Multiplex real-time PCR for the detection of Shiga Toxin-producing Escherichia coli in foods.- DNA isolation from cocoa-derived products and cocoa authentication by TaqMan real-time PCR.- Quantitative real-time PCR for the detection of allergenic species in foods.- Accurate absolute quantification of bacterial populations in mixed cultures by qPCR.- Real-time PCR method for assessment of ParA-mediated recombination efficiency in minicircle production.- Gene expression quantification from pathogenic bacterial biofilms by quantitative PCR.- A real-time quantitative PCR prool for the quantification of plasmid copy number in Lacoccus lactis.- Improved PCR by the use of disruptors, a new class of oligonucleotide reagents.- Mihondrial DNA D-loop amplification and sequencing for species differentiation in milk.- Long-range Polymerase Chain Reaction.- Megaprimer-based PCR to synthesise fusion genes for cloning.- Bacteria and Yeast ColonyPCR.- Inverse PCR for site-directed mutagenesis.- Optimized design of degenerate primers for PCR based on DNA or protein sequence comparisons.