Protocols for Nucleic Acid Analysis by Nonradioactive Probes

by Elena Hilario (Editor), John F. MacKay (Editor)

Overview

Prools for Nucleic Acid Analysis by Non-radioactive Probes, Second Edition provides a firm background on the basic preparative prools required for the analysis of nucleic acids by nonradioactive methods. Presenting the methodologies using amazing new applications, this volume offers guide chapters on nucleic acid extractions, preparation of nucleic acid blots, and labeling of nucleic acids with nonradioactive haptens. New fluorescent techniques such as Real Time PCR and microarrays are also included, allowing users to get a nonradioactive prool implemented in the laboratory with minimum adaptation required and fastest time to results.

The prools follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Product Details

ISBN-13:	9781588294302
Publisher:	Springer-Verlag New York, LLC
Publication date:	01/12/2007
Series:	Methods in Molecular Biology , #353
Edition description:	Second Edition 2007
Pages:	321
Product dimensions:	6.10(w) x 9.25(h) x 0.04(d)

Table of Contents

Nucleic Acid Extractions.- Genomic DNA Isolation From Different Biological Materials.- Extraction of Plant RNA.- Indirect Detection.- Overview of Hybridization and Detection Techniques.- Checkerboard DNA-DNA Hybridization Technology Using Digoxigenin Detection.- Nonradioactive Northern and Southern Analyses From Plant Samples.- Screening a BAC Library With Nonradioactive Overlapping Oligonucleotide (Overgo) Probes.- Direct In-Gel Hybridization of DNA With Digoxigenin-Labeled Probes.- In Situ Hybridization of Termite Microbes.- Fluorescent Labeling and Detection.- RNA Electrophoretic Mobility Shift Assay Using a Fluorescent DNA Sequencer.- Comparative Quantitation of mRNA Expression in the Central Nervous System Using Fluorescence In Situ Hybridization.- Visualization of Gene Expression by Fluorescent Multiplex Reverse Transcriptase-PCR Amplification.- Fluorescence In Situ Hybridization for the Identification of Environmental Microbes.- Kinetic (“Real-Time”) PCR.- to Kinetic (Real-Time) PCR.- Validation of Short Interfering RNA Knockdowns by Quantitative Real-Time PCR.- Real-Time Quantitative PCR as an Alternative to Southern Blot or Fluorescence In Situ Hybridization for Detection of Gene Copy Number Changes.- Design and Work-Up of a New Molecular Diagnostic Assay Based on Real-Time PCR.- Real-Time PCR Fluorescent Chemistries.- Microarrays.- Microarrays.- Oligonucleotide Microarrays for the Study of Coastal Microbial Communities.

Editorial Reviews

From the reviews of the second edition:

...this book provides a collection of good prools which are in general clearly written. They can be easily employed on the bench without being a specialist in a particular fields. - FEBS Letters

...Covers the basics of a variety of probe-labelling systems very well...a very interesting read. It could save you money and trouble from the radiation safety officer.-Biotechnology and Applied Biochemistry

"This is a set of prools for various types of nucleic acid analysis using nonradiocative methods. … The goal is to provide prools for nucleic acid analysis using other than radioactive reporter systems. This approach is commonplace at present, so a volume of practical utility has merit. … The target audience would be research laboratory personnel working in this general area. The editors have recruited a group of contributors with worldwide representation." (Eugene A Davidson, Doody’s Review Service, November, 2007)

"The editors of this volume have gathered together a variety of prools for anyone who wishes to use nonradioactive methods for the analysis of their chosen nucleic acid molecules. ... Combined with the excellent overviews on microarrays as well as hybridization and detection techniques, and several useful assays for the application of these techniques for nucleic acid analysis, scientists will find this book handy for their research and educators will adopt it as a useful teaching tool for advanced students." (Tzvi Tzfira, The Quarterly Review of Biology, Vol. 83, 2008)

From the Publisher