Radiation Oncology and Radiotherapy, Part A
Radiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more. Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2α phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of development, characterization and applications in CAR T Cells
1141903516
Radiation Oncology and Radiotherapy, Part A
Radiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more. Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2α phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of development, characterization and applications in CAR T Cells
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Radiation Oncology and Radiotherapy, Part A

Radiation Oncology and Radiotherapy, Part A

Radiation Oncology and Radiotherapy, Part A

Radiation Oncology and Radiotherapy, Part A

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Overview

Radiation Oncology and Radiotherapy, Part A, Volume 172 in the Methods in Cell Biology series, highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including DNA damage quantification by the COMET assay, Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cells, Immunoblotting-based characterization of the DNA damage response, Assessment of lipid peroxidation in irradiated cells, A simple method to assess clonogenic survival of irradiated cancer cells, Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescence, Cytofluorometric assessment of cell cycle progression in irradiated cells, and more. Other sections cover Assessment of transcription inhibition as a characteristic of immunogenic cell death, Assessment of eIF2α phosphorylation during immunogenic cell death, Quantification of cytosolic DNA species by immunofluorescence and automated image analysis, Flow cytometry-assisted quantification of CALR exposure during immunogenic cell death, Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cells, along with many additional topics of interest. - Provides the authority and expertise of leading contributors from an international board of authors - Presents the latest release in the Methods in Cell Biology series - Includes the latest information on the topic of development, characterization and applications in CAR T Cells

Product Details

ISBN-13: 9780323899505
Publisher: Elsevier Science & Technology Books
Publication date: 09/03/2022
Series: Methods in Cell Biology , #172
Sold by: Barnes & Noble
Format: eBook
Pages: 214
File size: 11 MB
Note: This product may take a few minutes to download.

About the Author

Ai Sato is a research specialist at Stich Radiation Oncology, New York, NY, USA
Jeffrey Kraynak is a research laboratory specialist at Stich Radiation Oncology, New York, NY, USA.
Dr. Ariel E. Marciscano is an assistant professor of Radiation Oncology at Weill Cornell Medical College.
Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Table of Contents

1. DNA damage quantification by the COMET assayMathias Weyland2. Immunofluorescence microscopy-assisted quantification of ATM and ATR activation in irradiated cellsIlio Vitale3. Immunoblotting-based characterization of the DNA damage responseIlio Vitale4. Assessment of lipid peroxidation in irradiated cellsBoyi Gan, Chao Mao, Amber Horbath and Guang Lei5. A simple method to assess clonogenic survival of irradiated cancer cellsMaria Esperanza Rodriguez Ruiz6. Quantification of beta-galactosidase activity as a marker of radiation-driven cellular senescenceGiulia Petroni7. Cytofluorometric assessment of cell cycle progression in irradiated cellsGiulia Petroni, Ai Sato, Aitziber Buqué Martínez and Lorenzo Galluzzi8. Assessment of transcription inhibition as a characteristic of immunogenic cell deathGuido Kroemer, Oliver Kepp, Giulia Cerrato and Allan Sauvat9. Assessment of eIF2α phosphorylation during immunogenic cell deathGuido Kroemer, Oliver Kepp and Lucillia Bezu10. Quantification of cytosolic DNA species by immunofluorescence and automated image analysisLorenzo Galluzzi11. Flow cytometry-assisted quantification of CALR exposure during immunogenic cell deathJitka Fucikova12. Interference of immunogenic anticancer therapy by artificially controlled calreticulin secretion from tumor cellsGuido Kroemer, Oliver Kepp, Peng LIU and Liwei Zhao13. Assessment of type I interferon responses  as a feature of immunogenic cell death.Guido Kroemer, Oliver Kepp, Sabrina Forveille, Allan Sauvat and Liwei Zhao14. Assessment of immunological memory formation in vivoGuido Kroemer, Oliver Kepp, Liwei Zhao and Peng LIU15. Characterization of the SASP of cancer cells experiencing DNA damageFrancis Rodier16. ELISA-based quantification of type I IFN secretion by irradiated cancer cellsClaire I. Vanpouille-Box17. RT-PCR-assisted quantification of type I IFN responses by irradiated cancer cellsLorenzo Galluzzi18. Methods to characterize the exosomal output of irradiated cancer cellsSheila Spada19. Cytofluorometric assessment of acute cell death responses driven by radiation therapyLorenzo Galluzzi

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